Expression pattern of T-helper 17 cell signaling pathway and mucosal inflammation in celiac disease
2014 (English)In: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, Vol. 49, no 2, 145-156 p.Article in journal (Refereed) Published
Objective. The aim was to investigate the mucosal activation of a broad range of genes associated with the T-helper 17 cell (Th17) signaling pathway in children at different stages of celiac disease (CD), including children with increased risk for CD and children with untreated and gluten-free diet (GFD)-treated CD. Material and methods. Small intestinal biopsies were taken from children with untreated and GFD-treated CD, transglutaminase antibody (TGA)-positive children with potential CD, and reference children. Real-time polymerase chain reaction (PCR) arrays were used to study the gene expression pattern of Th17-related genes, and quantitative PCR was used to study the interleukin (IL)-17A expression. Results. The mucosal expression of CD8A was elevated at all stages of CD. Children with untreated CD had diminished levels of IL-17RE, IL-23R, RORc, STAT6, CCL22, NFATC2, IL-18, CD4, CD247, and matrix metalloproteinase (MMP)9 but had elevated levels of MMP3, IL-17, interferon-gamma (IFN-gamma) and CD8A, compared to references. The majority of the aforementioned genes, being differentially expressed in untreated CD, displayed similar expression in GFD-treated children and references. Children with untreated and GFD-treated CD had elevated expression of IFN-gamma but had reduced expression of CD247. Interestingly, children with potential CD displayed reduced FOXP3, IL-21, and IL-17A levels. Conclusion. Mucosal upregulation of Th17 immunity occurs at the late stage of disease and is downregulated with dietary treatment, thus indicating that IL-17 immunity is not a fundamental feature of CD as Th1 immunity, which is not fully downregulated by GFD.
Place, publisher, year, edition, pages
Informa Healthcare , 2014. Vol. 49, no 2, 145-156 p.
arrays; celiac disease; children; gene expression; gluten-free diet; IL-17; mucosa; Th17
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-104288DOI: 10.3109/00365521.2013.863966ISI: 000329874800003OAI: oai:DiVA.org:liu-104288DiVA: diva2:697188