Change search
ReferencesLink to record
Permanent link

Direct link
Proteomic Amino-Termini Profiling Reveals Targeting Information for Protein Import into Complex Plastids
Show others and affiliations
2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 9, e74483- p.Article in journal (Refereed) Published
Abstract [en]

In organisms with complex plastids acquired by secondary endosymbiosis from a photosynthetic eukaryote, the majority of plastid proteins are nuclear-encoded, translated on cytoplasmic ribosomes, and guided across four membranes by a bipartite targeting sequence. In-depth understanding of this vital import process has been impeded by a lack of information about the transit peptide part of this sequence, which mediates transport across the inner three membranes. We determined the mature N-termini of hundreds of proteins from the model diatom Thalassiosira pseudonana, revealing extensive N-terminal modification by acetylation and proteolytic processing in both cytosol and plastid. We identified 63 mature N-termini of nucleus-encoded plastid proteins, deduced their complete transit peptide sequences, determined a consensus motif for their cleavage by the stromal processing peptidase, and found evidence for subsequent processing by a plastid methionine aminopeptidase. The cleavage motif differs from that of higher plants, but is shared with other eukaryotes with complex plastids.

Place, publisher, year, edition, pages
2013. Vol. 8, no 9, e74483- p.
National Category
Chemical Sciences
URN: urn:nbn:se:umu:diva-82292DOI: 10.1371/journal.pone.0074483ISI: 000324494000126OAI: diva2:697082
Available from: 2014-02-17 Created: 2013-10-29 Last updated: 2014-02-17Bibliographically approved

Open Access in DiVA

fulltext(1745 kB)154 downloads
File information
File name FULLTEXT01.pdfFile size 1745 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Schröder, Wolfgang P.
By organisation
Department of Chemistry
In the same journal
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 154 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 71 hits
ReferencesLink to record
Permanent link

Direct link