Methods for radiolabelling of monoclonal antibodies
2014 (English)In: Human Monoclonal Antibodies: Methods and Protocols / [ed] Michael Steinitz, Humana Press, 2014, 309-330 p.Chapter in book (Refereed)
The use of radionuclide labels allows to study the pharmacokinetics of monoclonal antibodies, to control the specificity of their targeting and to monitor the response to an antibody treatment with high accuracy. Selection of label depends on the processing of an antibody after binding to an antigen, and on the character of information to be derived from the study (distribution of antibody in the extracellular space, target occupancy or determination of sites of metabolism). This chapter provides protocols for labelling of antibodies with iodine-125 (suitable also for other radioisotopes of iodine) and with indium-111. Since radiolabelling might damage and reduce the immunoreactive fraction and/or affinity of an antibody, the methods for assessment of these characteristics of an antibody are provided for control.
Place, publisher, year, edition, pages
Humana Press, 2014. 309-330 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 1060
Radiology, Nuclear Medicine and Medical Imaging
IdentifiersURN: urn:nbn:se:uu:diva-218570DOI: 10.1007/978-1-62703-586-6_16PubMedID: 24037848ISBN: 978-1-62703-585-9ISBN: 978-1-62703-586-6OAI: oai:DiVA.org:uu-218570DiVA: diva2:695997