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Haemophilus parainfluenzae expresses diverse lipopolysaccharide O-antigens using ABC transporter and Wzy polymerase-dependent mechanisms
UCL, England University of Oxford, England .
Karolinska Institute, Sweden .
Karolinska Institute, Sweden .
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology. University of Oxford, England.
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2013 (English)In: International Journal of Medical Microbiology, ISSN 1438-4221, E-ISSN 1618-0607, Vol. 303, no 8, 603-617 p.Article in journal (Refereed) Published
Abstract [en]

Lipopolysaccharide O-antigens are the basis of serotyping schemes for Gram negative bacteria and help to determine the nature of host-bacterial interactions. Haemophilus parainfluenzae is a normal commensal of humans but is also an occasional pathogen. The prevalence, diversity and biosynthesis of O-antigens were investigated in this species for the first time. 18/18 commensal H. parainfluenzae isolates contain a O-antigen biosynthesis gene cluster flanked by glnA and pepB, the same position as the hmg locus for tetrasaccharide biosynthesis in Haemophilus influenzae. The O-antigen loci show diverse restriction digest patterns but fall into two main groups: (1) those encoding enzymes for the synthesis and transfer of Fuc-NAc4N in addition to the Wzy-dependent mechanism of O-antigen synthesis and transport and (2) those encoding galactofuranose synthesis/transfer enzymes and an ABC transporter. The other glycosyltransferase genes differ between isolates. Three H. parainfluenzae isolates fell outside these groups and are predicted to synthesise O-antigens containing ribitol phosphate or deoxytalose. Isolates using the ABC transporter system encode a putative O-antigen ligase, required for the synthesis of O-antigen-containing LPS glycoforms, at a separate genomic location. The presence of an O-antigen contributes significantly to H. parainfluenzae resistance to the killing effect of human serum in vitro. The discovery of O-antigens in H. parainfluenzae is striking, as its close relative H. influenzae lacks this cell surface component.

Place, publisher, year, edition, pages
Elsevier , 2013. Vol. 303, no 8, 603-617 p.
Keyword [en]
Lipopolysaccharide; O-antigen; Host-bacterial interaction; Cell surface; Pasteurellaceae
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-103880DOI: 10.1016/j.ijmm.2013.08.006ISI: 000328810000025OAI: oai:DiVA.org:liu-103880DiVA: diva2:692307
Available from: 2014-01-30 Created: 2014-01-30 Last updated: 2017-12-06

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