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Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Anaesthesiology and Intensive Care. Critical Care Medicine Research Group, Department of Intensive Care Unit, Tampere University Hospital, FI-33521 Tampere, Finland .
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2013 (English)In: Biology open, ISSN 2046-6390, Vol. 2, no 6, 605-612 p.Article in journal (Refereed) Published
Abstract [en]

The possibilities of human pluripotent stem cell-derived neural cells from the basic research tool to a treatment option in regenerative medicine have been well recognized. These cells also offer an interesting tool for in vitro models of neuronal networks to be used for drug screening and neurotoxicological studies and for patient/disease specific in vitro models. Here, as aiming to develop a reductionistic in vitro human neuronal network model, we tested whether human embryonic stem cell (hESC)-derived neural cells could be cultured in human cerebrospinal fluid (CSF) in order to better mimic the in vivo conditions. Our results showed that CSF altered the differentiation of hESC-derived neural cells towards glial cells at the expense of neuronal differentiation. The proliferation rate was reduced in CSF cultures. However, even though the use of CSF as the culture medium altered the glial vs. neuronal differentiation rate, the pre-existing spontaneous activity of the neuronal networks persisted throughout the study. These results suggest that it is possible to develop fully human cell and culture-based environments that can further be modified for various in vitro modeling purposes.

Place, publisher, year, edition, pages
2013. Vol. 2, no 6, 605-612 p.
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Medical and Health Sciences
URN: urn:nbn:se:uu:diva-216183DOI: 10.1242/bio.20134648ISI: 000209206300009PubMedID: 23789111OAI: diva2:689185
Available from: 2014-01-20 Created: 2014-01-20 Last updated: 2016-10-27Bibliographically approved

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Tenhunen, Jyrki
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