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A universal method for species identification of mammals utilizing next generation sequencing for the analysis of DNA mixtures
National Board of Forensic Medicine, Linköping, Sweden.
National Board of Forensic Medicine, Linköping, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. National Board of Forensic Medicine, Linköping, Sweden.
2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 12, e83761- p.Article in journal (Refereed) Published
Abstract [en]

Species identification can be interesting in a wide range of areas, for example, in forensic applications, food monitoring and in archeology. The vast majority of existing DNA typing methods developed for species determination, mainly focuses on a single species source. There are, however, many instances where all species from mixed sources need to be determined, even when the species in minority constitutes less than 1 % of the sample. The introduction of next generation sequencing opens new possibilities for such challenging samples. In this study we present a universal deep sequencing method using 454 GS Junior sequencing of a target on the mitochondrial gene 16S rRNA. The method was designed through phylogenetic analyses of DNA reference sequences from more than 300 mammal species. Experiments were performed on artificial species-species mixture samples in order to verify the method's robustness and its ability to detect all species within a mixture. The method was also tested on samples from authentic forensic casework. The results showed to be promising, discriminating over 99.9 % of mammal species and the ability to detect multiple donors within a mixture and also to detect minor components as low as 1 % of a mixed sample.

Place, publisher, year, edition, pages
Public Library of Science , 2013. Vol. 8, no 12, e83761- p.
National Category
Medical and Health Sciences
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URN: urn:nbn:se:liu:diva-103045DOI: 10.1371/journal.pone.0083761ISI: 000328735700137PubMedID: 24358309OAI: oai:DiVA.org:liu-103045DiVA: diva2:686216
Available from: 2014-01-10 Created: 2014-01-10 Last updated: 2017-12-06Bibliographically approved

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