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DegS and RseP Homologous Proteases Are Involved in Singlet Oxygen Dependent Activation of RpoE in Rhodobacter sphaeroides
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
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2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 11, e79520- p.Article in journal (Refereed) Published
Abstract [en]

Singlet oxygen (O-1(2)) is the main agent of photooxidative stress and is generated by photosensitizers as (bacterio) chlorophylls. It leads to the damage of cellular macromolecules and therefore photosynthetic organisms have to mount an adaptive response to O-1(2) formation. A major player of the photooxidative stress response in Rhodobacter sphaeroides is the alternative sigma factor RpoE, which is inactivated under non-stress conditions by its cognate anti-sigma factor ChrR. By using random mutagenesis we identified RSP_1090 to be required for full activation of the RpoE response under O-1(2) stress, but not under organic peroxide stress. In this study we show that both RSP_1090 and RSP_1091 are required for full resistance towards O-1(2). Moreover, we revealed that the DegS and RseP homologs RSP_3242 and RSP_2710 contribute to O-1(2) resistance and promote ChrR proteolysis. The RpoE signaling pathway in R. sphaeroides is therefore highly similar to that of Escherichia coli, although very different anti-sigma factors control RpoE activity. Based on the acquired results, the current model for RpoE activation in response to O-1(2) exposure in R. sphaeroides was extended.

Place, publisher, year, edition, pages
2013. Vol. 8, no 11, e79520- p.
National Category
Natural Sciences
URN: urn:nbn:se:uu:diva-212320DOI: 10.1371/journal.pone.0079520ISI: 000326597400070OAI: diva2:677657
Available from: 2013-12-10 Created: 2013-12-09 Last updated: 2013-12-10Bibliographically approved

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