Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Lactobacilli Suppress Gene Expression of Key Proteins Involved in miRNA Biogenesis in HT29 and VK2/E6E7 Cells
Örebro University, School of Science and Technology.
2013 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

It has previously been demonstrated that lactic acid bacteria are able to influence the innate immune response of host cells. One way this can be achieved is through modulation of inflammatory cascades initiated by pattern recognition elements such as toll-like receptors. Micro RNA can also have an effect on innate immunity, and has been shown to have an influence in regulation of these pathways in immune responsive cells. However, it is yet to be determined if the interaction between lactic acid bacteria and host cells involves regulation of the RNA interference machinery involved in micro RNA biogenesis. Three of the key proteins responsible for miRNA production and activation are Argonaute 2, Dicer and Drosha. Together, these are responsible for the processing and activation of miRNA to enable post-transcriptional gene regulation. In this study we have used quantitative PCR to evaluate changes in gene expression of these enzymes in HT29 and VK2/E6E7 mucosal epithelial cells after treatment with Lactobacillus and uropathogenic bacteria. We have found that bacterial treatment downregulates gene expression of elements responsible for miRNA biogenesis, and our results showed different responses dependent on the cell line. In addition to this we have also determined stable reference genes for use in further studies involving this model. Our findings indicate that modulation of the RNAi machinery might be an important element of immune regulation by bacterial colonists.

Place, publisher, year, edition, pages
2013. , 31 p.
Keyword [en]
Argonaute, Dicer, Drosha
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:oru:diva-32633OAI: oai:DiVA.org:oru-32633DiVA: diva2:675844
Subject / course
Biology
Supervisors
Examiners
Available from: 2013-12-05 Created: 2013-12-04 Last updated: 2017-10-17Bibliographically approved

Open Access in DiVA

fulltext(429 kB)264 downloads
File information
File name FULLTEXT01.pdfFile size 429 kBChecksum SHA-512
972d4b0326f9ab245c3388af906b9b352f23381dd0353077cd1b6188a912fde26c674a56ae35f0693df3c656e2c525d7e0cf575b13591370b7d1cefe84ca2a1f
Type fulltextMimetype application/pdf

By organisation
School of Science and Technology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 264 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 152 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf