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Amyloid Beta1-40-Induced Astrogliosis and the Effect of Genistein Treatment in Rat: A Three-Dimensional Confocal Morphometric and Proteomic Study
Ilam University of Medical Science, Iran .
Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Health Sciences.
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2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 10Article in journal (Refereed) Published
Abstract [en]

Astrocytes are highly involved in regulation and homeostasis of the extracellular environment in the healthy brain. In pathological conditions, these cells play a major role in the inflammatory response seen in CNS tissues, which is called reactive astrogliosis and includes hypertrophy and proliferation of astrocytes. Here, we performed 3D confocal microscopy to evaluate the morphological response of reactive astrocytes positive for glial fibrillary acidic protein (GFAP) in rats, to the presence of Aβ1–40 in the rat brain before and after treatment with genistein. In 50 astrocytes per animal, we measured the volume and surface area for the nucleus, cell body, the entire cell, the tissue covered by single astrocytes and quantified the number and length of branches, the density of the astrocytes and the intensity of GFAP immunoreactivity. Injecting Aβ1–40 into the brain of rats caused astrogliosis indicated by increased values for all measured parameters. Mass spectrometric analysis of hippocampal tissue in Aβ1–40-injected brain showed decreased amounts of tubulins, enolases and myelin basic protein, and increased amounts of dihydropyrimidinase-related protein 2. In Aβ1–40-injected rats pretreated with genistein, GFAP intensity was decreased to the sham-operated group level, and Aβ1–40-induced astrogliosis was significantly ameliorated.

Place, publisher, year, edition, pages
Public Library of Science , 2013. Vol. 8, no 10
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Medical and Health Sciences
URN: urn:nbn:se:liu:diva-101388DOI: 10.1371/journal.pone.0076526ISI: 000325810900075OAI: diva2:666311

Funding Agencies|County Council of Ostergotland (Sweden)||Linkoping University||Cellular and Molecular Research Center||Tehran University of Medical Sciences (Tehran, Iran)||

Available from: 2013-11-22 Created: 2013-11-21 Last updated: 2016-02-29

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Nyström, SofieTurkina, MariaHammarström, PerMohseni, Simin
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