Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis
2013 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 76, no 2, 236-246 p.Article in journal (Refereed) Published
Light-harvesting complex II (LHCII) contains three highly homologous chlorophyll-a/b-binding proteins (Lhcb1, Lhcb2 and Lhcb3), which can be assembled into both homo- and heterotrimers. Lhcb1 and Lhcb2 are reversibly phosphorylated by the action of STN7 kinase and PPH1/TAP38 phosphatase in the so-called state-transition process. We have developed antibodies that are specific for the phosphorylated forms of Lhcb1 and Lhcb2. We found that Lhcb2 is more rapidly phosphorylated than Lhcb1: 10sec of state 2 light' results in Lhcb2 phosphorylation to 30% of the maximum level. Phosphorylated and non-phosphorylated forms of the proteins showed no difference in electrophoretic mobility and dephosphorylation kinetics did not differ between the two proteins. In state 2, most of the phosphorylated forms of Lhcb1 and Lhcb2 were present in super- and mega-complexes that comprised both photosystem (PS)I and PSII, and the state 2-specific PSI-LHCII complex was highly enriched in the phosphorylated forms of Lhcb2. Our results imply distinct and specific roles for Lhcb1 and Lhcb2 in the regulation of photosynthetic light harvesting.
Place, publisher, year, edition, pages
Wiley-Blackwell, 2013. Vol. 76, no 2, 236-246 p.
Arabidopsis, Lhcb2, LHCII, phosphorylation, state transitions
IdentifiersURN: urn:nbn:se:umu:diva-83099DOI: 10.1111/tpj.12297ISI: 000325367300006OAI: oai:DiVA.org:umu-83099DiVA: diva2:664945
FunderSwedish Research Council
This work was supported by the European Union project FP7-PEO-PLE-ITN-2008 'HARVEST: Control of Light Use Efficiency in Plants and Algae From Light to Harvest', the Swedish Research Council (VR), the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas), the Academy of Finland (project Nos 118637 and 138703) and C. Leoni by a 1-year fellowship from the University of Bari (Rectoral Decree No. 1598). The authors declare no conflict of interest.2013-11-182013-11-182015-04-29Bibliographically approved