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Investigation of the Impact on Yeast Fermentation Performance in Production of Pale Lager Beer through Management Control
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
2013 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Utredning av påverkan på jästfermentering genom hanteringsstyrning vid produktion av ljus lageröl (Swedish)
Abstract [en]

Through a full factorial design experiment, the effects of time between worts, wort aeration and yeast dosage in production of a pale lager beer were examined in the beer process at Spendrups Bryggeri AB. The aim was to learn how different parameters may affect the yeast fermentation performance during beer production. Response variables used were the concentrations of ethyl acetate and isoamyl acetate, free amino nitrogen (FAN) degradation and change in extract. A statistical analysis showed that the concentration of ethyl acetate is dependent on yeast dosage and the interaction between time between worts and aeration while the isoamyl acetate concentration is dependent on yeast dosage and time between worts. No parameters are statistically significant for FAN degradation while the change in extract is dependent on the yeast dosage. Due to botched runs, mostly because of aeration problems, it was not possible to verify theoretical parameter values and responses. Since the aeration was not properly performed, the management of the aeration control should be further investigated. Ester analysis and analysis of FAN were performed as worts entered and exited horizontal fermentation tanks. An additional analysis of ester content was also performed as the early stage beer was transferred into lagering tanks. Cell viability as well as extract, pH and tank temperature was measured daily to verify the state of fermentation. Statistical calculations showed that when using NucleoCounter YC-100, there is no significant difference between analysis made of samples homogenized by a magnetic stirrer and samples shaken by hand.

Place, publisher, year, edition, pages
2013. , 46 p.
Keyword [en]
Yeast Fermentation, Full Factorial Design, Wort Aeration, Yeast Dosage, Ester Production, Yeast Viability
National Category
Bioprocess Technology
URN: urn:nbn:se:liu:diva-100150ISRN: LITH-IFM-A-EX--13/2839--SEOAI: diva2:660447
External cooperation
Spendrups Bryggeri AB
Subject / course
Available from: 2013-11-04 Created: 2013-10-29 Last updated: 2013-11-04Bibliographically approved

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