Age-Related Thinning of Bowman's Layer in the Human Cornea In Vivo
2013 (English)In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, Vol. 54, no 9, 6143-6149 p.Article in journal (Refereed) Published
Purpose. To determine the thickness of Bowman's layer (BL) in vivo in a healthy population and to determine its variation with age.
Methods. Eighty-two subjects aged 15 to 88 years with clear, healthy corneas were examined bilaterally with laser scanning in vivo confocal microscopy (IVCM). Bowman's layer thickness was determined from IVCM images of anterior and posterior BL boundaries. For a given eye, BL thickness was averaged across four central locations by two independent observers. In addition, central corneal thickness was measured by time-domain optical coherence tomography.
Results. A significant negative correlation of BL thickness with age was found in right eyes (Pearson r = −0.579, P < 0.0001) and in left eyes (r = −0.558, P < 0.0001). Linear regression analysis yielded a decline in BL thickness of 0.06 μm per year. In 41 older subjects (mean age, 64.4 years), BL thickness was significantly thinner (mean ± SD, 8.6 ± 1.7 μm in right eyes) than that in 41 younger subjects (mean age, 31.6 years) (mean ± SD, 10.7 ± 1.6 μm in right eyes) (P < 0.001). No correlation of corneal thickness with age or of BL thickness with corneal thickness was observed. Strong intereye correlations in BL thickness (r = 0.771, P < 0.0001) and corneal thickness (r = 0.969, P < 0.001) were found.
Conclusions. Bowman's layer thins with age in the normal cornea, losing one-third of its thickness between the ages of 20 and 80 years. In vivo measurement of BL thickness by IVCM could aid in clinical assessment and planned treatments of the anterior cornea.
Place, publisher, year, edition, pages
Association for Research in Vision and Ophthalmology (ARVO) , 2013. Vol. 54, no 9, 6143-6149 p.
Bowmans layer, in vivo confocal microscopy, image analysis, aging, collagen
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-100037DOI: 10.1167/iovs.13-12535ISI: 000325169500028OAI: oai:DiVA.org:liu-100037DiVA: diva2:659429
Funding Agencies|Swedish Research Council||Konung Gustav V and Drottning Viktorias Frimurarestiftelse||County Council of Ostergotland||Cronqvist Foundation||2013-10-252013-10-252014-04-30Bibliographically approved