Global differences in specific histone H3 methylation are associated with overweight and type 2 diabetes.
2013 (English)In: Clinical Epigenetics, E-ISSN 1868-7083, Vol. 5, no 1, 15Article in journal (Refereed) Published
BACKGROUND: Epidemiological evidence indicates yet unknown epigenetic mechanisms underlying a propensity for overweight and type 2 diabetes. We analyzed the extent of methylation at lysine 4 and lysine 9 of histone H3 in primary human adipocytes from 43 subjects using modification-specific antibodies.
RESULTS: The level of lysine 9 dimethylation was stable, while adipocytes from type 2 diabetic and non-diabetic overweight subjects exhibited about 40% lower levels of lysine 4 dimethylation compared with cells from normal-weight subjects. In contrast, trimethylation at lysine 4 was 40% higher in adipocytes from overweight diabetic subjects compared with normal-weight and overweight non-diabetic subjects. There was no association between level of modification and age of subjects.
CONCLUSIONS: The findings define genome-wide molecular modifications of histones in adipocytes that are directly associated with overweight and diabetes, and thus suggest a molecular basis for existing epidemiological evidence of epigenetic inheritance.
Place, publisher, year, edition, pages
BioMed Central, 2013. Vol. 5, no 1, 15
Endocrinology and Diabetes
IdentifiersURN: urn:nbn:se:liu:diva-99450DOI: 10.1186/1868-7083-5-15ISI: 000329455000001PubMedID: 24004477OAI: oai:DiVA.org:liu-99450DiVA: diva2:657156