Development and characterization of a brain tumor mimicking fluorescence phantom
2014 (English)In: Proceedings of SPIE, the International Society for Optical Engineering, ISSN 0277-786X, ISSN ISSN 0277-786X, Vol. 8945, no 894505, 1-6 p.Article in journal (Refereed) Published
Fluorescence guidance using 5-aminolevulinic acid (5-ALA) for brain tumor resection is a recent technique applied to the highly malignant brain tumors. Five-ALA accumulates as protoporphyrin IX fluorophore in the tumor cells in different concentrations depending on the tumor environment and cell properties. Our group has developed a fluorescence spectroscopy system used with a hand-held probe intra-operatively. The system has shown improvement of fluorescence detection and allows quantification that preliminarily correlates with tumor malignancy grade during surgery. However, quantification of fluorescence is affected by several factors including the initial fluorophore concentration, photobleaching due to operating lamps and attenuation from the blood. Accordingly, an optical phantom was developed to enable controlled fluorescence measurements and evaluation of the system outside of the surgical procedure. The phantom mimicked the optical properties of glioma at the specific fluorescence excitation wavelength when different concentrations of the fluorophore were included in the phantom. To allow evaluation of photobleaching, kinetics of fluorophore molecules in the phantom was restricted by solidifying the phantoms. Moreover, a model for tissue autofluorescence was added. The fluorescence intensity’s correlation with fluorophore concentration in addition to the photobleaching properties were investigated in the phantoms and were compared to the clinical data measured on the brain tumor.
Place, publisher, year, edition, pages
2014. Vol. 8945, no 894505, 1-6 p.
IdentifiersURN: urn:nbn:se:liu:diva-98989DOI: 10.1117/12.2039861ISI: 000334097200003ISBN: 9780819498588OAI: oai:DiVA.org:liu-98989DiVA: diva2:656052
Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue VI, 1-2 February 2014, San Francisco, California, USA