In Vitro Synthesis of Metabolites of three Anabolic Androgenic Steroids, by Human Liver Microsomes
Anabolic androgenic steroids are substances frequently misused to improve physical
performance in sports. To reveal substances misused as doping, athlete urine
samples are collected and tested. To identify the steroid and/or its metabolites
in urine, reference compounds must exist for comparison. The time-consuming
and ethical concerns about in vivo excretion studies for the examination of these
compounds, make the use of liver fragment microsomes an attractive alternative.
The aim of this thesis was to synthesize and identify metabolites from known and
rare anabolic androgenic steroids, by the use of human liver microsomes. Liver
is an important organ in steroid metabolism. By incubating AAS with human
liver microsomes and co factors, an in vitro simulation of the liver metabolism was
carried out. A conrmation of metabolites was performed by gas chromatography
tandem mass spectrometry in full scan, MRM mode, or both. 6beta-hydroxymethyl
metandienon, epimetandienon and 17,17-dimethylmetandienon were successfully
synthesized from metandienon, and the 17beta-hydroxymetandienon was produced
from the 17,17-dimethyl metabolite. Respectively three and one metabolite(s)
were found for the "designer steroids" methylnortestosteron and madol. Metabolite
variations were observed regarding the optimal time of incubation, and enzymatic
requirements of formation.
Place, publisher, year, edition, pages
Institutt for bioteknologi , 2013. , 90 p.
IdentifiersURN: urn:nbn:no:ntnu:diva-22910Local ID: ntnudaim:9364OAI: oai:DiVA.org:ntnu-22910DiVA: diva2:655060
Bruheim, Per, FørsteamanuensisHullstein, Ingunn