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In Vitro Synthesis of Metabolites of three Anabolic Androgenic Steroids, by Human Liver Microsomes
Norwegian University of Science and Technology, Faculty of Natural Sciences and Technology, Department of Biotechnology.
2013 (English)MasteroppgaveStudent thesis
Abstract [en]

Anabolic androgenic steroids are substances frequently misused to improve physical performance in sports. To reveal substances misused as doping, athlete urine samples are collected and tested. To identify the steroid and/or its metabolites in urine, reference compounds must exist for comparison. The time-consuming and ethical concerns about in vivo excretion studies for the examination of these compounds, make the use of liver fragment microsomes an attractive alternative. The aim of this thesis was to synthesize and identify metabolites from known and rare anabolic androgenic steroids, by the use of human liver microsomes. Liver is an important organ in steroid metabolism. By incubating AAS with human liver microsomes and co factors, an in vitro simulation of the liver metabolism was carried out. A conrmation of metabolites was performed by gas chromatography tandem mass spectrometry in full scan, MRM mode, or both. 6beta-hydroxymethyl metandienon, epimetandienon and 17,17-dimethylmetandienon were successfully synthesized from metandienon, and the 17beta-hydroxymetandienon was produced from the 17,17-dimethyl metabolite. Respectively three and one metabolite(s) were found for the "designer steroids" methylnortestosteron and madol. Metabolite variations were observed regarding the optimal time of incubation, and enzymatic requirements of formation.

Place, publisher, year, edition, pages
Institutt for bioteknologi , 2013. , 90 p.
URN: urn:nbn:no:ntnu:diva-22910Local ID: ntnudaim:9364OAI: diva2:655060
Available from: 2013-10-09 Created: 2013-10-09 Last updated: 2013-10-09Bibliographically approved

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