Jämförelse av resultat mellan manuell gelkortsanalys och automatisk immunohematologisk gelkortsanalys vid utförande av blodgruppskontroll och antikroppsscreening (BAS-test)
Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Incompatibility between donors and recipients can cause fatal hemolytic transfusion reactions (HTR), kidney damage and death and immunization of the recipient. Antigens on the surface of erythrocytes in the ABO blood group system and regular natural antibodies against these in the patient’s plasma must be considered. An individual always has a corresponding antibody of IgM in their plasma. Irregular antibodies acquired by transfusion or pregnancy and active in body temperature also cause the HTR incompatibility. Before blood transfusion a test is performed to investigate the patient’s ABO antigen and the antigen Rh D, and an antibody screen, a compability test called BAS-test. The test is manual performed using a gel card technique. Immunohematology 1000 (IH-1000) is a fully automatic computerized system that automatically performs the test and passes the results directly to the patient file. To investigate whether these methods provide comparable results blood sample from the vein from a total of 118 blood donors and patients (50 men and 68 women) in the ages of 16-99 years were collected. Both manual gel card technique and analysis with the IH-1000 were performed. The results showed a good agreement (100%) between the two techniques for both affiliation of ABO of erythrocytes and findings of irregular antibodies in plasma. The patients who received a positive antibody screen with the manual technique also received a positive antibody screen with erythrocytes from same test donor in the analysis of IH-1000. Between the two methods no clinically significant difference was observed in the antibody response in the strength of the reactions. BAS-test can be performed with IH-1000 instead of manually performed technique.
Place, publisher, year, edition, pages
2013. , 15 p.
manuell gelkortsanalys, automatisk immunohematologisk gelkortsanalys, blodgruppskontroll, antikroppsscreening
Biomedical Laboratory Science/Technology
IdentifiersURN: urn:nbn:se:lnu:diva-29185OAI: oai:DiVA.org:lnu-29185DiVA: diva2:653149
Subject / course
Biomedical Laboratory Science Programme, 180 credits
Rydén, Ingvar, MD, PhDWidell, Susanne, Dr Med Vet.
Mattson, Maria, Dr Med Vet.