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Mutational analysis of the csgD mRNA leader: search for a mode of regulation
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2013 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The CsgD protein is the master regulator of a pathway leading to the formation of curli, in essence regulating the switch between a motile and a sessile lifestyle for bacteria. The 5’-UTR region of the csgD mRNA is a hotspot for multiple regulatory small RNAs (sRNA) involved in a complex regulatory network. Even though it is previously known how the interaction takes place it is unknown how sRNA binding affects the translational activity. In order to suggest a mode of regulation a mutational assay was performed by making changes in the csgD 5’-UTR and investigate what the translational effects were. Mutations in different regions are shown to affect the translation levels in various ways.

Place, publisher, year, edition, pages
2013. , 45 p.
Keyword [en]
Regulatory small RNA, enterobacteria, CsgD, OmrA, OmrB, mutational analysis, in vivo and in vitro translation assay
National Category
Microbiology Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-208415OAI: diva2:652458
Educational program
Molecular Biotechnology Engineering Programme
Available from: 2013-09-30 Created: 2013-09-30 Last updated: 2013-09-30Bibliographically approved

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MicrobiologyBiochemistry and Molecular Biology

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