Mutational analysis of the csgD mRNA leader: search for a mode of regulation
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
The CsgD protein is the master regulator of a pathway leading to the formation of curli, in essence regulating the switch between a motile and a sessile lifestyle for bacteria. The 5’-UTR region of the csgD mRNA is a hotspot for multiple regulatory small RNAs (sRNA) involved in a complex regulatory network. Even though it is previously known how the interaction takes place it is unknown how sRNA binding affects the translational activity. In order to suggest a mode of regulation a mutational assay was performed by making changes in the csgD 5’-UTR and investigate what the translational effects were. Mutations in different regions are shown to affect the translation levels in various ways.
Place, publisher, year, edition, pages
2013. , 45 p.
Regulatory small RNA, enterobacteria, CsgD, OmrA, OmrB, mutational analysis, in vivo and in vitro translation assay
Microbiology Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-208415OAI: oai:DiVA.org:uu-208415DiVA: diva2:652458
Molecular Biotechnology Engineering Programme
Wagner, Gerhart, Prof.