Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
iNOS-Dependent Increase in Colonic Mucus Thickness in DSS-Colitic Rats
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Show others and affiliations
2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 8, e71843- p.Article in journal (Refereed) Published
Abstract [en]

Aim: To investigate colonic mucus thickness in vivo in health and during experimental inflammatory bowel disease. Methods: Colitis was induced with 5% DSS in drinking water for 8 days prior to experiment, when the descending colonic mucosa of anesthetized rats was studied using intravital microscopy. Mucus thickness was measured with micropipettes attached to a micromanipulator. To assess the contributions of NOS and prostaglandins in the regulation of colonic mucus thickness, the non-selective NOS-inhibitor L-NNA (10 mg/kg bolus followed by 3 mg/kg/h), the selective iNOS-inhibitor L-NIL (10 mg/kg bolus followed by 3 mg/kg/h) and the non-selective COX-inhibitor diclofenac (5 mg/kg) were administered intravenously prior to experiment. To further investigate the role of iNOS in the regulation of colonic mucus thickness, iNOS -/- mice were used. Results: Colitic rats had a thicker firmly adherent mucus layer following 8 days of DSS treatment than untreated rats (88 +/- 2 mu m vs 76 +/- 1 mu m). During induction of colitis, the thickness of the colonic mucus layer initially decreased but was from day 3 significantly thicker than in untreated rats. Diclofenac reduced the mucus thickness similarly in colitic and untreated rats (-16 +/- 5 mu m vs -14 +/- 2 mu m). While L-NNA had no effect on colonic mucus thickness in DSS or untreated controls (+3 +/- 2 mm vs +3 +/- 1 mu m), L-NIL reduced the mucus thickness significantly more in colitic rats than in controls (-33 +/- 4 mu m vs -10 +/- 3 mu m). The importance of iNOS in regulating the colonic mucus thickness was confirmed in iNOS-/- mice, which had thinner colonic mucus than wild-type mice (35 +/- 3 mu m vs 50 +/- 2 mu m, respectively). Furthermore, immunohistochemistry revealed increased levels of iNOS in the colonic surface epithelium following DSS treatment. Conclusion: Both prostaglandins and nitric oxide regulate basal colonic mucus thickness. During onset of colitis, the thickness of the mucus layer is initially reduced followed by an iNOS mediated increase.

Place, publisher, year, edition, pages
2013. Vol. 8, no 8, e71843- p.
National Category
Medical and Health Sciences Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-208083DOI: 10.1371/journal.pone.0071843ISI: 000323425700106OAI: oai:DiVA.org:uu-208083DiVA: diva2:650835
Available from: 2013-09-23 Created: 2013-09-23 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

fulltext(5887 kB)340 downloads
File information
File name FULLTEXT01.pdfFile size 5887 kBChecksum SHA-512
3634d9a31970b5d918e93d77c36b5dbfa32fe1375021270253b28fc3e50258a6a593cf04d1be293c5b82749c1ff73d838f0ff8e269758cd1f89755674ad71e13
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Walden, TomasAhl, DavidSandler, StellanPhillipson, MiaHolm, Lena
By organisation
Department of Medical Cell Biology
In the same journal
PLoS ONE
Medical and Health SciencesNatural Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 340 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 679 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf