Quantification of lipid accumulation in the diaphragm after mechanical ventilation
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
During mechanical ventilation the diaphragm experiences an extreme case of muscleunloading. In many cases this results in respiratory muscle dysfunctions making it difficult towean the patient off the ventilator. One component in this dysfunction is the accumulation ofintramyocellular lipids (IMCL) in the diaphragm muscle fibres. Using Oil Red O stainingsand confocal microscopy on rat diaphragm sections we have quantified this process. Theresults show a sudden increase in IMCL contents between 18 and 24 hours. No significantdifference between fibre types could be seen.
Place, publisher, year, edition, pages
2013. , 25 p.
lipid accumulation, diaphragm, mechanical ventilation, IMCL, oil red, ICU, intensive care unit, Sprague-Dawley, rat, ATPase staining, confocal microscopy
Biochemistry and Molecular Biology Anesthesiology and Intensive Care Biomedical Laboratory Science/Technology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:uu:diva-206940OAI: oai:DiVA.org:uu-206940DiVA: diva2:646285
Molecular Biotechnology Engineering Programme
Larsson, Lars, professor
Josefsson, Lars-Göran, utb.samordnare