Productivity and salinity structuring of the microplankton revealed by comparative freshwater metagenomics
2014 (English)In: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 16, no 9, 2682-2698 p.Article in journal (Refereed) Published
Little is known about the diversity and structuring of freshwater microbial communities beyond the patterns revealed by tracing their distribution in the landscape with common taxonomic markers such as the ribosomal RNA. To address this gap in knowledge, metagenomes from temperate lakes were compared to selected marine metagenomes. Taxonomic analyses of rRNA genes in these freshwater metagenomes confirm the previously reported dominance of a limited subset of uncultured lineages of freshwater bacteria, whereas Archaea were rare. Diversification into marine and freshwater microbial lineages was also reflected in phylogenies of functional genes and there were also significant differences in functional beta-diversity. The pathways and functions that accounted for these differences are involved in osmoregulation, active transport, carbohydrate and amino acid metabolism. Moreover, predicted genes orthologous to active transporters and recalcitrant organic matter degradation were more common in microbial genomes from oligotrophic versus eutrophic lakes. This comparative metagenomic analysis allowed us to formulate a general hypothesis that oceanic- compared to freshwater-dwelling microorganisms, invest more in metabolism of amino acids and that strategies of carbohydrate metabolism differ significantly between marine and freshwater microbial communities.
Place, publisher, year, edition, pages
2014. Vol. 16, no 9, 2682-2698 p.
Microbiology Natural Sciences
IdentifiersURN: urn:nbn:se:uu:diva-206205DOI: 10.1111/1462-2920.12301ISI: 000341579700006PubMedID: 24118837OAI: oai:DiVA.org:uu-206205DiVA: diva2:645061
Published in 2013 as Epub ahead of print2013-09-032013-08-292014-10-29Bibliographically approved