The Structural Basis for Optimal Performance of Oligothiophene-Based Fluorescent Amyloid Ligands: Conformational Flexibility is Essential for Spectral Assignment of a Diversity of Protein Aggregates
2013 (English)In: Chemistry - A European Journal, ISSN 0947-6539, E-ISSN 1521-3765, Vol. 19, no 31, 10179-10192 p.Article in journal (Refereed) Published
Protein misfolding diseases are characterized by deposition of protein aggregates, and optical ligands for molecular characterization of these disease-associated structures are important for understanding their potential role in the pathogenesis of the disease. Luminescent conjugated oligothiophenes (LCOs) have proven useful for optical identification of a broader subset of disease-associated protein aggregates than conventional ligands, such as thioflavin T and Congo red. Herein, the molecular requirements for achieving LCOs able to detect nonthioflavinophilic Aβ aggregates or non-congophilic prion aggregates, as well as spectrally discriminate Aβ and tau aggregates, were investigated. An anionic pentameric LCO was subjected to chemical engineering by: 1) replacing thiophene units with selenophene or phenylene moieties, or 2) alternating the anionic substituents along the thiophene backbone. In addition, two asymmetric tetrameric ligands were generated. Overall, the results from this study identified conformational freedom and extended conjugation of the conjugated backbone as crucial determinants for obtaining superior thiophene-based optical ligands for sensitive detection and spectral assignment of disease-associated protein aggregates.
Place, publisher, year, edition, pages
Wiley-VCH Verlag , 2013. Vol. 19, no 31, 10179-10192 p.
Alzheimers disease, fluorescent probes, luminescent conjugated oligothiophenes, microscopy, protein folding
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-96708DOI: 10.1002/chem.201301463ISI: 000321983700020OAI: oai:DiVA.org:liu-96708DiVA: diva2:642973
Funding Agencies|Swedish Foundation for Strategic Research||European Research Council||NIH|P50AG056815PO1-AG03991|2013-08-232013-08-232014-04-08