Characterization of the Transcription Factor NF‐Y in the Regulation of Zona Pellucida Genes in Zebrafish Ovary
Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Zona pellucida glycoproteins (ZP) are important proteins for maturation of the oocytes in eukaryotes, these proteins are encoded by cluster of zp genes. zp2.3 and zp3.5 genes are expressed during the developing and maturation of the oocytes in zebrafish ovaries. Both of the gens have a CCAAT box in their promoter regions, playing a big role in the expression of the both genes in zebrafish oocytes. The transcription of the genes in the eukaryotes requires transcription factors to initiate and promote the transcription, the transcription factors can bind to the promoter region and initiate the transcription process. The nuclear factor y (NFY) regulates the genes by binding to the CCAAT boxes in their promoter regions, it consist from many subunit such as NF-YA and NF-YB. Here in this study we characterize the expression pattern of NF-YA and NF-YB by screening these genes expression in several organs and tissues, also to determine its roles in the expression of the zp2.3 and zp3.5 genes in the adultzebrafish ovary.
Place, publisher, year, edition, pages
2011. , 25 p.
CCAAT box, expression pattern, promoter
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:oru:diva-30318OAI: oai:DiVA.org:oru-30318DiVA: diva2:642827
Subject / course
, Orebro (English)
Olsson, Per Erik, Professor