Evaluation of flow cytometry as replacement for plating in in vitro measurements of competitive growth under antibiotic stress
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
A method for measuring cell concentration and identity based on flow cytometry (FCM) and fluorescent marking is developed and subsequently compared with traditional plating based methods, with regards to performance, economy and ergonomy. The emphasis is on competitive growth of bacteria under antibiotic stress, but the technique could be used in any situation requiring fast, high throughput counting and identification of cellular populations. The method needs further development, but shows potential as a parallelizable and fast alternative to plating.
Place, publisher, year, edition, pages
2013. , 44 p.
UPTEC X, 13 003
Flow cytometry, competetive growth, antibiotics
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:uu:diva-204707OAI: oai:DiVA.org:uu-204707DiVA: diva2:639728
Molecular Biotechnology Engineering Programme
Lagerbäck, Pernilla, Dr.
Hughes, Diarmaid, Professor