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Evaluation of flow cytometry as replacement for plating in in vitro measurements of competitive growth under antibiotic stress
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Infectious Diseases.
2013 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

A method for measuring cell concentration and identity based on flow cytometry (FCM) and fluorescent marking is developed and subsequently compared with traditional plating based methods, with regards to performance, economy and ergonomy. The emphasis is on competitive growth of bacteria under antibiotic stress, but the technique could be used in any situation requiring fast, high throughput counting and identification of cellular populations. The method needs further development, but shows potential as a parallelizable and fast alternative to plating.

Place, publisher, year, edition, pages
2013. , 44 p.
Series
UPTEC X, 13 003
Keyword [en]
Flow cytometry, competetive growth, antibiotics
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-204707OAI: oai:DiVA.org:uu-204707DiVA: diva2:639728
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2013-08-09 Created: 2013-08-09 Last updated: 2013-08-09Bibliographically approved

Open Access in DiVA

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