Active liquid degassing in microfluidic systems
2013 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 13, no 22, 4366-4373 p.Article in journal, Editorial material (Refereed) Published
We present a method for efficient air bubble removal in microfluidic applications. Air bubbles are extracted from a liquid chamber into a vacuum chamber through a semipermeable membrane, consisting of PDMS coated with amorphous Teflon (R) AF 1600. Whereas air is efficiently extracted through the membrane, water loss is greatly reduced by the Teflon even at elevated temperatures. We present the water loss and permeability change with the amount of added Teflon AF to the membrane. Also, we demonstrate bubble-free, multiplex DNA amplification using PCR in a PDMS microfluidic device.
Place, publisher, year, edition, pages
RSC Publishing, 2013. Vol. 13, no 22, 4366-4373 p.
Air Bubble Formation, Pcr, Devices, Chip, Channels, Removal, Valves, Trap, Flow
IdentifiersURN: urn:nbn:se:kth:diva-125061DOI: 10.1039/c3lc50778eISI: 000325946800014ScopusID: 2-s2.0-84886056493OAI: oai:DiVA.org:kth-125061DiVA: diva2:639319
FunderEU, FP7, Seventh Framework Programme
QC 201311142013-08-062013-08-062015-06-18Bibliographically approved