Characterization of solutecarrier SLC38A6
Student paper other, 10 credits / 15 HE creditsStudent thesis
Transport across the membrane of a cell is of crucial importance for cellular functions. The solute carrier family,SLC38 is a family of membrane proteins that transports various substances through the membrane and thusperforms many physiologically important functions, for example, transport of glutamine from astrocyte toneurons in the central nervous system. In this paper, we demonstrate that one of the transporters in this familynamed SLC38A6 forms several protein complexes with a variety of proteins in the membrane and in synapticvesicles, suggesting that SLC38A6 is involved in the synaptic release of neurotransmitters in synapses. Weperformed sensitive protein interaction analysis between the protein of interest and a variety of proteinsexpressed at different sites in the neuronal cell. We showed that SLC38A6 interacts with proteins in the cellmembrane as well as in the membrane of synaptic vesicles. The current theory is that SLC38A6 interact withthese proteins when the synaptic vesicles are in close proximity with the cell membrane during the release of theneurotransmitters.
Place, publisher, year, edition, pages
2012. , 11 p.
Solute carriers, SLC38A6, PLA, proximity ligation assay, Molecular biotechnology engineering programme, Department of Neuroscience, Functional Pharmacology, Uppsala University, Research training, forskningspraktik, civilingenjör molekylär bioteknik, membrane transport, membrane proteins, synaptic vesicles, protein-protein interactions, ImageTool, Somar Al-walai
Natural Sciences Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-203329OAI: oai:DiVA.org:uu-203329DiVA: diva2:636141
Molecular Biotechnology Engineering Programme