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The effects of coating structure and water-holding capacity on the oxygen-scavenging ability of enzymes embedded in the coating layer
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.
Energy and Process Engineering, Tampere University of Technology.
Karlstad University, Faculty of Technology and Science, Paper Surface Centre. Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.ORCID iD: 0000-0001-7368-7227
Kemiska institutionen, Department of Chemistry, Umeå Universitet.
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2013 (English)In: TAPPI Journal, ISSN 0734-1415, Vol. 12, no 6, p. 43-52Article in journal (Refereed) Published
Abstract [en]

Enzymes catalyzing oxygen scavenging were embedded in latex-based coatings with and without barrier kaolin clay to produce material for active packages. The clay was used to create a porous structure, and the closed-structure matrix consisted of a biopolymer comprising either starch or gelatin to increase the water uptake of the coating. The effects of the porous open structure and of the water uptake of the coated layer on the oxygen-scavenging ability of the embedded enzymes were examined at both 75% and 100% relative humidity. The results showed that the porous clay structure led to higher oxygen-scavenging capacity than that of a closed structure at both test conditions by enabling a high diffusion rate for oxygen and glucose to the active sites of the enzymes. The addition of a water-holding biopolymer did not always significantly affect the oxygen-scavenging capacity. However for a less-porous layer at 100% relative humidity, an increase in the amount of biopolymer resulted in an increase in oxygen-scavenging capacity. The results were treated statistically using multiple-factor analysis where the most important factor for the oxygen-scavenging ability was found to be the addition of clay. The coatings were also characterized with respect to water vapor uptake, overall migration, porosity, and scanning electron microscopy images.

Place, publisher, year, edition, pages
TAPPI Press, 2013. Vol. 12, no 6, p. 43-52
Keywords [en]
Active packaging, oxygen scavenging, enzymes, coating
National Category
Chemical Engineering Industrial Biotechnology
Research subject
Chemical Engineering
Identifiers
URN: urn:nbn:se:kau:diva-28458ISI: 000321046600005OAI: oai:DiVA.org:kau-28458DiVA, id: diva2:635953
Available from: 2013-07-08 Created: 2013-07-08 Last updated: 2017-12-06Bibliographically approved
In thesis
1. Oxygen-reducing enzymes in coatings and films for active packaging
Open this publication in new window or tab >>Oxygen-reducing enzymes in coatings and films for active packaging
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Oxygen scavengers are used in active packages to protect the food against deteriorative oxidation processes. The aim of this work was to investigate the possibilities to produce oxygen-scavenging packaging materials based on oxygen-reducing enzymes. The enzymes were incorporated into a dispersion coating formulation applied onto a food-packaging board using conventional laboratory coating techniques.

Various enzymes were used: a glucose oxidase, an oxalate oxidase and three laccases originating from different organisms. All of the enzymes were successfully incorporated into a coating layer and could be reactivated after drying. For at least two of the enzymes, re-activation was possible not only by using liquid water but also by using water vapour. Re-activation of the glucose oxidase and a laccase required relative humidities of greater than 75% and greater than 92%, respectively.

Catalytic reduction of oxygen gas by glucose oxidase was promoted by creating an open structure through addition of clay to the coating at a level above the critical pigment volume concentration. Migration of the enzyme and the substrate was reduced by adding an extrusion-coated liner of polypropylene on top of the coating.

For the laccase-catalysed reduction of oxygen it was possible to use lignin derivatives as substrates for the enzymatic reaction. The laccase-catalysed reaction created a polymeric network by cross-linking of lignin-based entities, which resulted in increased stiffness and increased water-resistance of biopolymer films. The laccases were also investigated with regard to their potential to function as oxygen scavengers at low temperatures. At 7°C all three laccases retained more than 20% of the activity they had at room temperature (25°C), which suggests that the system is also useful for packaging of refrigerated food.

Place, publisher, year, edition, pages
Karlstad: Karlstads universitet, 2013. p. 91
Series
Karlstad University Studies, ISSN 1403-8099 ; 2013:38
Keywords
Active packaging, food packaging, oxygen scavengers, oxygen-reducing enzymes, dispersion coating, biopolymers
National Category
Chemical Engineering
Research subject
Chemical Engineering
Identifiers
urn:nbn:se:kau:diva-28749 (URN)978-91-7063-516-8 (ISBN)
Public defence
2013-10-18, 9C204, Rejmersalen, Karlstad, 10:15 (English)
Opponent
Supervisors
Available from: 2013-09-27 Created: 2013-08-27 Last updated: 2014-10-27Bibliographically approved

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