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New Procedure for Predictions of Overloaded Profiles in Gradient Elution
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Chemical Sciences.ORCID iD: 0000-0001-8561-6872
Rzeszow University of Technology.
Karlstad University, Faculty of Health, Science and Technology (starting 2013), Department of Engineering and Physics.ORCID iD: 0000-0002-8943-6286
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2013 (English)Conference paper, Published paper (Other academic)
Abstract [en]

To simulate the separation process in liquid chromatography, the competitive adsorption isotherms need to be known. In gradient elution, the adsorption isotherms are determined with isocratic experiments on different mobile-phase plateaus, levels covering the range used in the gradient program. This can lead to extreme retention times for some mobile-phase compositions and therefore it might even be impossible to determine all necessary adsorption data using the traditional isocratic approach. In this talk, we will present a method where single and competitive nonlinear adsorption isotherms are determined directly from overloaded elution profiles in gradient elution. The numerical coefficients in the adsorption isotherms are determined by the inverse method that minimizes the difference between calculated and experimental elution profiles. This is a new method where the need for tedious/impossible isocratic experiments is eliminated. The method is systematically verified using both synthetic and experimental data. Finally the new method is used to successfully predict elution profiles for a two-component mixture in gradient elution. The new method open up the opportunity to study the adsorption of substances whose retention factor vary strongly with the mobile-phase composition, like peptides and proteins, where the classic methods will fail. We also intend to transfer the metholology for SFC in near future; but there are some problems to be solved first (see our SFC posters). This is a contribution from the Fundamental Separation Science Group www.FSSG.se

Place, publisher, year, edition, pages
Amsterdam, 2013.
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kau:diva-28111OAI: oai:DiVA.org:kau-28111DiVA, id: diva2:631185
Conference
39th International Symposium on High Performance Liquid Phase Separations and Related Techniques, AMSTERDAM: June 16 - 20, 2013
Available from: 2013-06-20 Created: 2013-06-20 Last updated: 2017-12-06Bibliographically approved

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Fornstedt, TorgnyÅsberg, DennisEnmark, MartinForssén, PatrikSamuelsson, Jörgen
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