Analysis of the response of Lactococcus lactis towards sublethal alcohol concentrations
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
In this study, I analyzed the Lactococcus lactis subspecies cremoris MG1363 stress response at sub-lethal alcohol levels during exponential growth phase at transcriptomics, proteomics, metabolomics levels. Ethanol, 1-butanol, 1-hexanol were the selected alcohols. Manganese- transporter- and arginine catabolic pathway genes were up-regulated by all alcohols suggesting they evoked oxidative and acidic stress. ATP manganese transporter genes, histidine- and galactose genes were also up-regulated. Purine- and pyrimidine synthesis genes were down-regulated. HPLC analysis displayed decreased biomass yield and glycolytic flux, suggesting increased glycolytic energy production and slowed down overall enzymatic rate. Proteomics analysis displayed differential expressed proteins associated with heat and oxidative stress.
Place, publisher, year, edition, pages
2013. , 65 p.
UPTEC X, 13 006
L. lactis, growth experiments, alcohol stress, transcriptomics, proteomics
Genetics Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-201442OAI: oai:DiVA.org:uu-201442DiVA: diva2:627287
Molecular Biotechnology Engineering Programme
Kilstrup, Mogens, Professor
Wagner, Gerhart, Professor