Change search
ReferencesLink to record
Permanent link

Direct link
Analysis of the response of Lactococcus lactis towards sublethal alcohol concentrations
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2013 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

In this study, I analyzed the Lactococcus lactis subspecies cremoris MG1363 stress response at sub-lethal alcohol levels during exponential growth phase at transcriptomics, proteomics, metabolomics levels. Ethanol, 1-butanol, 1-hexanol were the selected alcohols. Manganese- transporter- and arginine catabolic pathway genes were up-regulated by all alcohols suggesting they evoked oxidative and acidic stress. ATP manganese transporter genes, histidine- and galactose genes were also up-regulated. Purine- and pyrimidine synthesis genes were down-regulated. HPLC analysis displayed decreased biomass yield and glycolytic flux, suggesting increased glycolytic energy production and slowed down overall enzymatic rate. Proteomics analysis displayed differential expressed proteins associated with heat and oxidative stress.

Place, publisher, year, edition, pages
2013. , 65 p.
UPTEC X, 13 006
Keyword [en]
L. lactis, growth experiments, alcohol stress, transcriptomics, proteomics
National Category
Genetics Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-201442OAI: diva2:627287
Educational program
Molecular Biotechnology Engineering Programme
Available from: 2013-06-11 Created: 2013-06-11 Last updated: 2013-06-11Bibliographically approved

Open Access in DiVA

fulltext(4139 kB)397 downloads
File information
File name FULLTEXT01.pdfFile size 4139 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
Biology Education Centre
GeneticsBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 397 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 244 hits
ReferencesLink to record
Permanent link

Direct link