Neuronal Differentiation and Extensive Migration of Human Neural Precursor Cells following Co-Culture with Rat Auditory Brainstem Slices
2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 3Article in journal (Refereed) Published
Congenital or acquired hearing loss is often associated with a progressive degeneration of the auditory nerve (AN) in the inner ear. The AN is composed of processes and axons of the bipolar spiral ganglion neurons (SGN), forming the connection between the hair cells in the inner ear cochlea and the cochlear nuclei (CN) in the brainstem (BS). Therefore, replacement of SGNs for restoring the AN to improve hearing function in patients who receive a cochlear implantation or have severe AN malfunctions is an attractive idea. A human neural precursor cell (HNPC) is an appropriate donor cell to investigate, as it can be isolated and expanded in vitro with maintained potential to form neurons and glia. We recently developed a post-natal rodent in vitro auditory BS slice culture model including the CN and the central part of the AN for initial studies of candidate cells. Here we characterized the survival, distribution, phenotypic differentiation, and integration capacity of HNPCs into the auditory circuitry in vitro. HNPC aggregates (spheres) were deposited adjacent to or on top of the BS slices or as a monoculture (control). The results demonstrate that co-cultured HNPCs compared to monocultures (1) survive better, (2) distribute over a larger area, (3) to a larger extent and in a shorter time-frame form mature neuronal and glial phenotypes. HNPC showed the ability to extend neurites into host tissue. Our findings suggest that the HNPC-BS slice co-culture is appropriate for further investigations on the integration capacity of HNPCs into the auditory circuitry.
Place, publisher, year, edition, pages
Public Library of Science , 2013. Vol. 8, no 3
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-93403DOI: 10.1371/journal.pone.0057301ISI: 000318334500015OAI: oai:DiVA.org:liu-93403DiVA: diva2:624469
Funding Agencies|Swedish Research Council|2008-2822|Marianne and Marcus Wallenbergs Foundation||Petrus and Augusta Hedlunds Foundation||Swedish Association of Hard of Hearing People||Acta Otolaryngologicas Foundation||Foundation Tysta Skolan||Ollie and Elof Ericssons Foundation for Medical Research||Karolinska Institutet Foundations||Medical faculty||Lund University||2013-05-312013-05-312013-11-19