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Chemical and bioanalytical characterisation of PAHs in risk assessment of remediated PAH-contaminated soils
Örebro University, School of Science and Technology. (MTM)
Örebro University, School of Science and Technology. (MTM)ORCID iD: 0000-0003-4128-8226
Örebro University, School of Science and Technology. (MTM)
Örebro University, School of Science and Technology. (MTM)ORCID iD: 0000-0001-6217-8857
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2013 (English)In: Environmental science and pollution research international, ISSN 0944-1344, E-ISSN 1614-7499, Vol. 20, no 12, p. 8511-8520Article in journal (Refereed) Published
Abstract [en]

Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in soil at former industrial areas; and in Sweden, some of the most contaminated sites are being remediated. Generic guideline values for soil use after so-called successful remediation actions of PAH-contaminated soil are based on the 16 EPA priority pollutants, which only constitute a small part of the complex cocktail of toxicants in many contaminated soils. The aim of the study was to elucidate if the actual toxicological risks of soil samples from successful remediation projects could be reflected by chemical determination of these PAHs. We compared chemical analysis (GC-MS) and bioassay analysis (H4IIE-luc) of a number of remediated PAH-contaminated soils. The H4IIE-luc bioassay is an aryl hydrocarbon (Ah) receptor-based assay that detects compounds that activate the Ah receptor, one important mechanism for PAH toxicity. Comparison of the results showed that the bioassay-determined toxicity in the remediated soil samples could only be explained to a minor extent by the concentrations of the 16 priority PAHs. The current risk assessment method for PAH-contaminated soil in use in Sweden along with other countries, based on chemical analysis of selected PAHs, is missing toxicologically relevant PAHs and other similar substances. It is therefore reasonable to include bioassays in risk assessment and in the classification of remediated PAH-contaminated soils. This could minimise environmental and human health risks and enable greater safety in subsequent reuse of remediated soils.

Place, publisher, year, edition, pages
2013. Vol. 20, no 12, p. 8511-8520
National Category
Natural Sciences
Research subject
Biology; Environmental Chemistry; Enviromental Science
Identifiers
URN: urn:nbn:se:oru:diva-29084DOI: 10.1007/s11356-013-1787-6ISI: 000327498600022OAI: oai:DiVA.org:oru-29084DiVA, id: diva2:622275
Funder
Knowledge FoundationSwedish Environmental Protection AgencyAvailable from: 2013-05-21 Created: 2013-05-21 Last updated: 2017-12-06Bibliographically approved
In thesis
1. Chemical and bioanalytical characterisation of PAH-contaminated soils: identification, availability and mixture toxicity of AhR agonists
Open this publication in new window or tab >>Chemical and bioanalytical characterisation of PAH-contaminated soils: identification, availability and mixture toxicity of AhR agonists
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Contaminated soils are a worldwide problem. Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in soil at former industrial areas, especially at old gasworks sites, gas stations and former wood impregnation facilities. Risk assessments of PAHs in contaminated soils are usually based on chemical analysis of a small number of individual PAHs, which only constitute a small part of the complex cocktail of hundreds of PAHs and other related polycyclic aromatic compounds (PACs) in the soils. Generally, the mixture composition of PAH-contaminated soils is rarely known and the mechanisms of toxicity and interactions between the pollutants are far from fully understood.

The main objective of this thesis was to characterize remediated PAHcontaminated soils by use of a chemical and bioanalytical approach. Bioassay specific relative potency (REP) values for 38 PAHs and related PACs were developed in the sensitive H4IIE-luc bioassay and used in massbalance analysis of remediated PAH contaminated soils, to assess the contribution of chemically quantified compounds to the overall aryl hydrocarbon receptor (AhR)-mediated activity observed in the H4IIE-luc bioassay. Mixtures studies showed additive AhR-mediated effects of PACs, including PAHs, oxy PAHs, methylated PAHs and azaarenes, in the bioassay, which supports the use of REP values in risk assessment. The results from the chemical and bioassay analysis showed that PAH-contaminated soils contained a large fraction of AhR activating compounds whose effect could not be explained by chemical analysis of the 16 priority PAHs. Further chemical identification and biological studies are necessary to determine whether these unknown substances pose a risk to human health or the environment. Results presented in this thesis are an important step in the development of AhR-based bioassay analysis and risk assessment of complex PAH-contaminated samples.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2013. p. 60
Series
Örebro Studies in Chemistry, ISSN 1651-4270 ; 13
Keywords
Polycyclic aromatic compounds, Soil, Risk assessment, Mixture studies, AhR-mediated activity, REPs, GC/MS, H4IIE-luc bioassay
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:oru:diva-30070 (URN)978-91-7668-961-5 (ISBN)
Public defence
2013-09-27, Hörsalen, Billbergska huset, Örebro universitet, Fakultetsgatan 1, 701 82 Örebro, 10:00 (Swedish)
Opponent
Supervisors
Funder
Swedish Environmental Protection AgencyKnowledge Foundation
Note

Other funders: Sparbanksstiftelsen Nya and Ångpanneföreningen

Available from: 2013-07-30 Created: 2013-07-30 Last updated: 2017-10-17Bibliographically approved

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