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Pre- and post-natal Lactobacillus reuteri supplementation decreases allergen responsiveness in infancy
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
2013 (English)In: Clinical and Experimental Allergy, ISSN 0954-7894, E-ISSN 1365-2222, Vol. 43, no 4, p. 434-442Article in journal (Refereed) Published
Abstract [en]

Background: We have previously shown that Lactobacillus reuteri supplementation from pregnancy week 36 and to the infant through the first year of life decreased the prevalence of IgE-associated eczema at 2years. The underlying immunological mechanisms are unknown, however.

Objective: To investigate the immunomodulatory effect of probiotic supplementation on allergen- and mitogen-induced immune responses in children until 2years of age.

Methods: Blood mononuclear cells were collected at birth, 6, 12 and 24months from 61 children (29 probiotic and 32 placebo treated) and cultured with ovalbumin, birch and cat extract and Phytohaemagglutinin (PHA). Cytokine and chemokine secretion was determined using an in-house multiplexed Luminex assay and ELISA. Real-time PCR was performed to investigate the Ebi3, Foxp3, GATA-3 and T-bet mRNA expression.

Results: Probiotic treatment was associated with low cat-induced Th2-like responses at 6months (IL-5, P=0.01, and IL-13, P=0.009), with a similar trend for IL-5 at 12months (P=0.09). Cat-induced IFN- responses were also lower after probiotic than after placebo treatment at 24months (P=0.007), with similar findings for the anti-inflammatory IL-10 at birth (P=0.001) and at 12months (P=0.009). At 24months, Th2-associated CCL22 levels were lower in the probiotic than in the placebo group after birch stimulation (P=0.02), with a similar trend after ovalbumin stimulation (P=0.07). Lower CCL22 levels were recorded at 12 and 24months (P=0.03 and P=0.01) after PHA stimulation.

Conclusion and Clinical Relevance: Lactobacillus reuteri supplementation decreases allergen responsiveness and may enhance immunoregulatory capacity during infancy. L. reuteri supplementation from week 36 and during the first year of life significantly decreases IgE-associated eczema and lowers allergen and mitogen responsiveness.

Place, publisher, year, edition, pages
2013. Vol. 43, no 4, p. 434-442
Keyword [en]
allergen, allergy, chemokine, cytokine, eczema, Foxp3, infancy, Lactobacillus reuteri, probiotics, sensitization
National Category
Medical and Health Sciences
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-28901DOI: 10.1111/cea.12082ISI: 000316623800008OAI: oai:DiVA.org:oru-28901DiVA, id: diva2:619691
Available from: 2013-05-06 Created: 2013-05-03 Last updated: 2017-12-06Bibliographically approved

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