Change search
ReferencesLink to record
Permanent link

Direct link
Amperometric Biosensor for estimation of Glucose-6-phosphate Using Prussian Blue Nanoparticles.
Department of Polymer Science and Technology, University of Calcutta, India.
Department of Polymer Science and Technology, University of Calcutta, India.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. (Biosensors & Bioelectronics)ORCID iD: 0000-0002-1815-9699
2013 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 439, no 2, 194-200 p.Article in journal (Refereed) Published
Abstract [en]

Glucose-6-phosphateplays an important role in carbohydrate metabolism of all living organisms.Compared to the conventional analytical methods available for estimation of glucose-6-phosphate,the biosensors having relative simplicity, specificity, low-cost and fastresponse time are a promising alternative. We have reported a glucose-6-phosphatesensor based on screen-printed electrode utilizing Prussian blue nanoparticlesand enzymes, glucose-6-phosphate dehydrogenase and glutathione reductase. The Prussianblue nanoparticles acted as a mediator enhancing the rate of electrochemical responses.The Fourier transforminfrared spectroscopy and energy-dispersiveX-ray spectroscopy study confirmed the formation of Prussian blue, whereas, the atomic forced microscopy revealed that Prussian bluenanoparticles were about 25-30 nm in diameter. To obtainmaximum amperometric response, optimization studies were conducted for pH,enzyme and cofactor loading. The proposed glucose-6-phosphate biosensor showed goodstability, rapid response time and broad linear response in the range of 0.01-1.25mM and detection limit of 6.3 mM. The biosensor also worked well for serum samples and exhibitedexcellent anti-interference ability.

Place, publisher, year, edition, pages
2013. Vol. 439, no 2, 194-200 p.
Keyword [en]
Glucose-6-phosphate; Prussian blue nanoparticles; Screen-printed electrode; Amperometry
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-91855DOI: 10.1016/j.ab.2013.04.025ISI: 000322286900017OAI: diva2:619344
Available from: 2013-05-03 Created: 2013-05-03 Last updated: 2014-11-24

Open Access in DiVA

G6P(151 kB)902 downloads
File information
File name FULLTEXT01.pdfFile size 151 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Turner, Anthony
By organisation
Biosensors and BioelectronicsThe Institute of Technology
In the same journal
Analytical Biochemistry
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 902 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 238 hits
ReferencesLink to record
Permanent link

Direct link