Amperometric Biosensor for estimation of Glucose-6-phosphate Using Prussian Blue Nanoparticles.
2013 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 439, no 2, 194-200 p.Article in journal (Refereed) Published
Glucose-6-phosphateplays an important role in carbohydrate metabolism of all living organisms.Compared to the conventional analytical methods available for estimation of glucose-6-phosphate,the biosensors having relative simplicity, specificity, low-cost and fastresponse time are a promising alternative. We have reported a glucose-6-phosphatesensor based on screen-printed electrode utilizing Prussian blue nanoparticlesand enzymes, glucose-6-phosphate dehydrogenase and glutathione reductase. The Prussianblue nanoparticles acted as a mediator enhancing the rate of electrochemical responses.The Fourier transforminfrared spectroscopy and energy-dispersiveX-ray spectroscopy study confirmed the formation of Prussian blue, whereas, the atomic forced microscopy revealed that Prussian bluenanoparticles were about 25-30 nm in diameter. To obtainmaximum amperometric response, optimization studies were conducted for pH,enzyme and cofactor loading. The proposed glucose-6-phosphate biosensor showed goodstability, rapid response time and broad linear response in the range of 0.01-1.25mM and detection limit of 6.3 mM. The biosensor also worked well for serum samples and exhibitedexcellent anti-interference ability.
Place, publisher, year, edition, pages
2013. Vol. 439, no 2, 194-200 p.
Glucose-6-phosphate; Prussian blue nanoparticles; Screen-printed electrode; Amperometry
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-91855DOI: 10.1016/j.ab.2013.04.025ISI: 000322286900017OAI: oai:DiVA.org:liu-91855DiVA: diva2:619344