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Theoretical prediction of the protein-protein interaction between Arabidopsis thaliana COP1 and UVR8
University of Gothenburg, Gothenburg, Sweden.
University of Gothenburg, Gothenburg, Sweden.
Örebro University, School of Science and Technology. (Biokemi)ORCID iD: 0000-0003-3315-8835
2013 (English)In: Theoretical Chemistry accounts, ISSN 1432-881X, E-ISSN 1432-2234, Vol. 132, no 7, article id UNSP 1371Article in journal (Refereed) Published
Abstract [en]

In plants, ultraviolet-B radiation (280-315 nm) regulates gene expression and plant morphology through the UV RESPONSE LOCUS 8 (UVR8) photoreceptor. The first signaling event after quantal absorbance is the interaction of the UVR8 C-terminus with the E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1). The nature of the interaction between these two proteins is hitherto unknown. A protein homology model of the Arabidopsis thaliana COP1 seven bladed propeller WD40 repeat domain and de novo folds of the C-terminal 27 amino acid (amino acids 397-423) peptide of Arabidopsis UVR8 (UVR8397-423) is herein reported. Using a theoretical computational docking protocol, the interaction between COP1 and UVR8 was predicted. A core motif was identified in UVR8397-423 comprising adjacent hydrophobic residues V410 and P411 together with a charged residue D412, homologous to corresponding motifs in other COP1-binding proteins, such as ELONGATED HYPOCOTYL 5 (HY5), cryptochrome 1 (CRY1), and salt tolerance proteins STO/STH. The protein-protein interaction between the COP1 WD40 repeat domain and UVR8397-423 reveal binding within a region of COP1 overlapping the binding site for HY5 and the other COP1-interacting proteins. This study provides a framework for understanding docking between UVR8 and COP1, which in turn gives clues for experimental testing of UVR8/COP1 interaction.

Place, publisher, year, edition, pages
Springer, 2013. Vol. 132, no 7, article id UNSP 1371
Keywords [en]
Arabidopsis thaliana, COP1, Docking, Modeling, Protein-protein interaction, UVR8, WD40
National Category
Physical Chemistry Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:oru:diva-28871DOI: 10.1007/s00214-013-1371-7ISI: 000322174500001Scopus ID: 2-s2.0-84877088330OAI: oai:DiVA.org:oru-28871DiVA, id: diva2:618721
Projects
Plant UV-B Photobiology
Funder
Swedish Research Council
Note

Funding Agency:

Faculty of Science at University of Gothenburg 

Faculty of Business, Science and Technology at Örebro University 

Available from: 2013-04-29 Created: 2013-04-29 Last updated: 2018-05-17Bibliographically approved

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