Change search
ReferencesLink to record
Permanent link

Direct link
3D Microstructural Architecture of Muscle Attachments in Extant and Fossil Vertebrates Revealed by Synchrotron Microtomography
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Evolution and Developmental Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology.
Show others and affiliations
2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 2, e56992- p.Article in journal (Refereed) Published
Abstract [en]

Background: Firm attachments binding muscles to skeleton are crucial mechanical components of the vertebrate body. These attachments (entheses) are complex three-dimensional structures, containing distinctive arrangements of cells and fibre systems embedded in the bone, which can be modified during ontogeny. Until recently it has only been possible to obtain 2D surface and thin section images of entheses, leaving their 3D histology largely unstudied except by extrapolation from 2D data. Entheses are frequently preserved in fossil bones, but sectioning is inappropriate for rare or unique fossil material.

Methodology/Principal Findings: Here we present the first non-destructive 3D investigation, by propagation phase contrast synchrotron microtomography (PPC-SR mu CT), of enthesis histology in extant and fossil vertebrates. We are able to identify entheses in the humerus of the salamander Desmognathus from the organization of bone-cell lacunae and extrinsic fibres. Statistical analysis of the lacunae differentiates types of attachments, and the orientation of the fibres, reflect the approximate alignment of the muscle. Similar histological structures, including ontogenetically related pattern changes, are perfectly preserved in two 380 million year old fossil vertebrates, the placoderm Compagopiscis croucheri and the sarcopterygian fish Eusthenopteron foordi.

Conclusions/Significance: We are able to determine the position of entheses in fossil vertebrates, the approximate orientation of the attached muscles, and aspects of their ontogenetic histories, from PPC-SRmCT data. Sub-micron microtomography thus provides a powerful tool for studying the structure, development, evolution and palaeobiology of muscle attachments.

Place, publisher, year, edition, pages
2013. Vol. 8, no 2, e56992- p.
National Category
Natural Sciences
URN: urn:nbn:se:uu:diva-197999DOI: 10.1371/journal.pone.0056992ISI: 000315561400020OAI: diva2:614988
EU, European Research Council, 233111Knut and Alice Wallenberg Foundation
Available from: 2013-04-08 Created: 2013-04-08 Last updated: 2014-02-20Bibliographically approved

Open Access in DiVA

fulltext(2415 kB)233 downloads
File information
File name FULLTEXT01.pdfFile size 2415 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Sanchez, SophieRyll, BettinaWretman, LovisaAhlberg, Per E.
By organisation
Evolution and Developmental BiologyDepartment of Organismal Biology
In the same journal
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 233 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 328 hits
ReferencesLink to record
Permanent link

Direct link