Production and cleavage specificity determination of serine proteases mMCP-4, mMCP-5, rMCP-2 and two platypus serine proteases of the chymase locus.
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Serine proteases are a family of enzymes with a wide array of functions across both eukaryotes and prokaryotes. Here we have attempted to produce the serine proteases rat mast cell protease 2 and mouse mast cell protease 5 in a culture of HEK 293 cells; and mouse mast cell protease 4, platypus granzyme B-like protease and platypus hypothetical protease in a baculovirus expression system. Following production we wanted to analyse these serine proteases using a phage display assay and a battery of chromogenic substrates.
Place, publisher, year, edition, pages
2013. , 55 p.
Serine protease, substrate, hek 293, baculovirus, phage display, chromogenic substrates
IdentifiersURN: urn:nbn:se:uu:diva-197088OAI: oai:DiVA.org:uu-197088DiVA: diva2:611640
Molecular Biotechnology Engineering Programme
UppsokLife Earth Science
Hellman, Lars, Professor
Josefsson, Lars-Göran, Student faculty coordinator