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Attachment of macromolecular heparin conjugate to gelatin scaffolds improves endothelial cell infiltration
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
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2013 (English)In: Tissue Engineering. Parts A, B and C, ISSN 2152-4947, E-ISSN 2152-4955, Vol. 19, no 11-12, 1336-1348 p.Article in journal (Refereed) Published
Abstract [en]

Long-term survival of implanted cells requires oxygen and nutrients, the need for which is met by vasculari- zation of the implant. The use of scaffolds with surface-attached heparin as anchoring points for angiogenic growth factors has been reported to improve this process. We examined the potential role of surface modification of gelatin scaffolds in promoting endothelial cell infiltration by using a unique macromolecular conjugate of heparin as a coating. Compared to other heparin coatings, this surface modification provides flexible heparin chains, representing a new concept in heparin conjugation. In vitro cell infiltration of scaffolds was assessed using a three-dimensional model in which the novel heparin surface, without growth factors, showed a 2.5-fold increase in the number of infiltrating endothelial cells when compared to control scaffolds. No additional improvement was achieved by adding growth factors (vascular endothelial growth factor and/or fibroblast growth factor-2) to the scaffold. In vivo experiments confirmed these results and also showed that the addition of angiogenic growth factors did not significantly increase the endothelial cell infiltration but increased the number of inflammatory cells in the implanted scaffolds. The endothelial cell-stimulating ability of the heparin surface alone, combined with its growth factor-binding capacity, renders it an interesting candidate surface treatment to create a prevascularized site prepared for implantation of cells and tissues, in particular those sensitive to inflammation but in need of supportive revascularization, such as pancreatic islets of Langerhans.

Place, publisher, year, edition, pages
2013. Vol. 19, no 11-12, 1336-1348 p.
Keyword [en]
Endothelial cell infiltration, inflammatory cells, heparin coating, gelatin scaffold, growth factors
National Category
Cell and Molecular Biology
Research subject
Medical Cell Biology
Identifiers
URN: urn:nbn:se:uu:diva-195102DOI: 10.1089/ten.TEA.2011.0712ISI: 000318173800008PubMedID: 23327585OAI: oai:DiVA.org:uu-195102DiVA: diva2:606926
Funder
Swedish Research Council
Note

De två sista författarna delar sistaförfattarskapet.

Available from: 2013-02-21 Created: 2013-02-21 Last updated: 2017-12-06Bibliographically approved

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Publisher's full textPubMedhttp://online.liebertpub.com/doi/abs/10.1089/ten.TEA.2011.0712

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Brännström, JohanLarsson, RolfNilsson, BoMagnusson, Peetra

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