The Francisella tularensis LVS ΔpdpC mutant exhibits a unique phenotype during intracellular infection
2013 (English)In: BMC Microbiology, ISSN 1471-2180, E-ISSN 1471-2180, Vol. 13, 20Article in journal (Refereed) Published
Background: A prerequisite for the virulence of the facultative intracellular bacterium Francisella tularensis is effective intramacrophage proliferation, which is preceded by phagosomal escape into the cytosol, and ultimately leads to host cell death. Many components essential for the intracellular life cycle are encoded by a gene cluster, the Francisella pathogenicity island (FPI), constituting a type VI secretion system.
Results: We characterized the FPI mutant ΔpdpC of the live vaccine strain (LVS) of F. tularensis and found that it exhibited lack of intracellular replication, incomplete phagosomal escape, and marked attenuation in the mouse model, however, unlike a phagosomally contained FPI mutant, it triggered secretion of IL-1β, albeit lower than LVS, and markedly induced LDH release.
Conclusions: The phenotype of the ΔpdpC mutant appears to be unique compared to previously described F. tularensis FPI mutants.
Place, publisher, year, edition, pages
BioMed Central, 2013. Vol. 13, 20
Francisella tularensis, type VI secretion, cytopathogenicity, intracellular replication, PdpC
Microbiology Microbiology in the medical area
IdentifiersURN: urn:nbn:se:umu:diva-66129DOI: 10.1186/1471-2180-13-20ISI: 000314827600002PubMedID: 23356941OAI: oai:DiVA.org:umu-66129DiVA: diva2:605721
The Francisella tularensis LVS Delta pdpC mutant exhibits a unique phenotype during intracellular infection2013-02-152013-02-152016-05-24Bibliographically approved