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Variation of eubacterial and denitrifying bacterial biofilm communities among constructed wetlands
Department of Chemical Ecology/Ecotoxicology, Lund University, Lund, Sweden.
Halmstad University, School of Business and Engineering (SET), Biological and Environmental Systems (BLESS), Wetland Research Centre.
Halmstad University, School of Business and Engineering (SET), Biological and Environmental Systems (BLESS), Wetland Research Centre.ORCID iD: 0000-0002-5181-0391
Department of Chemical Ecology/Ecotoxicology, Lund University, Lund, Sweden.
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Abstract [en]

Bacteria play important roles in the transformation of nutrients in wetlands, but few studies have examined parameters affecting variation in bacterial community composition between wetlands. We compared the composition of eubacterial and denitrifying bacterial biofilm communities in 32 agricultural constructed wetlands in southern Sweden, and the extent to which wetland environmental parameters could explain the observed variation. Structure and richness of the eubacterial 16S rRNA gene and three denitrifying bacterial enzyme genes (nirK, nirS and nosZ), analysed by molecular fingerprinting methods, varied among the constructed wetlands, which could be partly explained by different environmental parameters. Results from the enzyme gene analyses were also compared to determine whether the practice of using a single denitrifying bacterial gene could characterize the overall community composition of denitrifying bacteria. We found that nirK was more diverse than both nirS and the nosZ, and the band structure and richness of the three genes were not related to the sam environmental parameters. This suggests that using a single enzyme gene may not suffice to characterize the community composition of denitrifying bacteria in constructed agricultural wetlands.

Keyword [en]
16S rDNA, nirK, nirS, nosZ, bacterial community composition, environmental parameters, PCR-DGGE, denitrification
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Natural Sciences
Identifiers
URN: urn:nbn:se:hh:diva-21484OAI: oai:DiVA.org:hh-21484DiVA: diva2:605290
Note

Included in doctoral thesis: Milenkovski, Susann. Structure and Function of Microbial Communities in Constructed Wetlands - Influence of environmental parameters and pesticides on denitrifying bacteria. Lund University 2009.

Available from: 2013-02-13 Created: 2013-02-13 Last updated: 2013-11-15Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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