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Conserved function of core clock proteins in the gymnosperm Norway spruce (Picea abies L. Karst)
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Plant Ecology and Evolution.
Växtbiologi och skogsgenetik, Plant Biology and Forest Genetics.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Plant Ecology and Evolution.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Plant Ecology and Evolution.
2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 3, e60110- p.Article in journal (Refereed) Published
Abstract [en]

From studies of the circadian clock in the plant model species Arabidopsis (Arabidopsis thaliana), a number of important properties and components have emerged. These include the genes CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), GIGANTEA (GI), ZEITLUPE (ZTL) and TIMING OF CAB EXPRESSION 1 (TOC1 also known as PSEUDO-RESPONSE REGULATOR 1 (PRR1)) that via gene expression feedback loops participate in the circadian clock. Here, we present results from ectopic expression of four Norway spruce (Picea abies) putative homologs (PaCCA1, PaGI, PaZTL and PaPRR1) in Arabidopsis, their flowering time, circadian period length, red light response phenotypes and their effect on endogenous clock genes were assessed. For PaCCA1-ox and PaZTL-ox the results were consistent with Arabidopsis lines overexpressing the corresponding Arabidopsis genes. For PaGI consistent results were obtained when expressed in the gi2 mutant, while PaGI and PaPRR1 expressed in wild type did not display the expected phenotypes. These results suggest that protein function of PaCCA1, PaGI and PaZTL are at least partlyconserved compared to Arabidopsis homologs, however further studies are needed to reveal the protein function of PaPRR1. Our data suggest that components of thethree-loop network typical of the circadian clock in angiosperms were present beforethe split of gymnosperms and angiosperms.

Place, publisher, year, edition, pages
2013. Vol. 8, no 3, e60110- p.
Keyword [en]
circadian clock, Picea abies (Norway spruce), transformants, gymnosperm, ZEITLUPE (ZTL), GIGANTEA (GI), TIMING OF CAB1 (TOC1 or PRR1), CIRCADIAN CLOCK ASSOCIATED 1 (CCA1)
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-192151DOI: 10.1371/journal.pone.0060110ISI: 000317262200084OAI: diva2:588972
Available from: 2013-01-16 Created: 2013-01-16 Last updated: 2013-05-22Bibliographically approved
In thesis
1. Genetic Control of Annual Growth Rhythm in the Conifer Norway Spruce (Picea Abies L. Karst)
Open this publication in new window or tab >>Genetic Control of Annual Growth Rhythm in the Conifer Norway Spruce (Picea Abies L. Karst)
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Norway spruce (Picea abies L. Karst) is a conifer belonging to the group gymnosperms and is an ecologically and economically important species in several parts of Europe. It is crucial for trees like Norway spruce to adapt timing of events such as bud set and growth cessation to the local environment in order to maximize the growth period while avoiding frost damage.

This thesis aims at widening the knowledge about genetic control of annual growth rhythm in Norway spruce and particularly the control of bud set. Using spruce transformants ectopically expressing PaFT/TFL1-LIKE 2 (PaFTL2) the prior hypothesis that PaFTL2 induces bud set is confirmed. This is further supported by spatial and temporal expression patterns in seedlings and adult trees. It is further shown that gymnosperms possess at least two FLOWERING LOCUS T/TERMINAL FLOWER 1 (FT/TFL1)-like genes with TFL1-like function, suggesting the ancestor of FT and TFL1 to be more TFL1-like. PaFTL1 appears to have complementary expression patterns to that of PaFTL2 both spatially and temporally indicating they may act together to control growth in Norway spruce.

Since bud set is controlled by photoperiod and circadian clock genes are implicated in this process, putative clock homologs were studied to gain insight into the circadian clock in gymnosperms. Several clock homologs were identified and their expression showed a diurnal pattern but the expression was rapidly damped in constant conditions. Transgenic Arabidopsis expressing putative core clock genes from spruce indicate that at least three genes, PaCCA1, PaGI and PaZTL, appear to have a conserved function between angiosperms and gymnosperms. Taken together these results suggest that gymnosperms have a similar core clock structure as angiosperms even though fundamental differences might exist since the cycling of the clock genes were rapidly damped in free-running conditions.

The studies presented in this thesis support substantial conservation of pathway components controlling photoperiodic responses in angiosperms and gymnosperms and identify PaFTL2 as a component of growth rhythm control. However, important changes in these processes are also evident. The results provide a solid basis for future research on molecular mechanisms controlling an adaptive trait in an important non-model organism.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2013. 49 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1014
bud set, circadian clock, Picea abies, growth cessation, phosphatidyl ethanolamine-binding protein (PEBP) genes
National Category
Plant Biotechnology Biochemistry and Molecular Biology
Research subject
Biology with specialization in Evolutionary Functional Genomics
urn:nbn:se:uu:diva-192180 (URN)978-91-554-8581-8 (ISBN)
Public defence
2013-03-01, Lindahlsalen, EBC, Norbyvägen 18B, Uppsala, 10:00 (English)
Available from: 2013-02-08 Created: 2013-01-16 Last updated: 2013-04-02Bibliographically approved

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Karlgren, AnnaKällman, ThomasLagercrantz, Ulf
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