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Physical and functional properties of chromatography media: a down-scale study
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2013 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

During down scale of column chromatography a phenomenon called wall effect appears.This supports the gel bed and gives it better flow properties. Using three different media infive different sized columns this effect was investigated regarding the physical andfunctional properties of the media. The conclusion of this study is that the effect appears incolumns below 26 mm in diameter and that the impact of the wall effect is larger the morerigid the used media is.

Place, publisher, year, edition, pages
2013. , 40 p.
Keyword [en]
Chromatography, down scale, wall effect, ion exchange, chromatography media
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:uu:diva-191749OAI: oai:DiVA.org:uu-191749DiVA: diva2:586903
External cooperation
GE Healthcare
Educational program
Molecular Biotechnology Engineering Programme
Uppsok
Technology
Supervisors
Examiners
Available from: 2013-01-13 Created: 2013-01-13 Last updated: 2013-01-13Bibliographically approved

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