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An Fcγ receptor I (CD64)-negative subpopulation of human peripheral blood monocytes is resistant to killing by antigen-activated CD4-positive cytotoxic T cells
Forschungszentrum Borstel, Department of Immunology and Cell Biology, Borstel, Germany.
Jagiellonian University, Institute of Molecular Biology, Cracow, Poland.
Forschungszentrum Borstel, Department of Immunology and Cell Biology, Borstel, Germany.
Deutsches Krebsforschungs-zentrum, Heidelberg, Germany.ORCID iD: 0000-0001-9518-1411
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1997 (English)In: European Journal of Immunology, ISSN 0014-2980, E-ISSN 1521-4141, Vol. 27, no 9, 2358-2365 p.Article in journal (Refereed) Published
Abstract [en]

It has been demonstrated that in monocyte/T cell co-cultures activated with recall antigens, cytotoxic T cells were generated which are able to reduce the number of antigen-presenting monocytes. In previous studies we could show that a minor subset of monocytes, the Fc gamma receptor I-negative (CD64(-)) monocytes, exhibits significantly higher antigen-presenting capacity than the main population of monocytes (> 90%) which are Fc gamma receptor I-positive (CD64(+)). Therefore, we addressed the question whether they are also differentially susceptible to T cell-mediated killing. In the present study we demonstrate that the CD64(-) monocyte subset is more resistant to killing by antigen-activated T cells than CD64(+) monocytes, as indicated by a higher viability and recovery of CD64(-) monocytes. This mechanism involves CD95 (Fas) antigen, since monocyte death in co-cultures with antigen-activated T cells could be partially reduced by blocking anti-Fas monoclonal antibodies (mAb). In agreement with this finding, although CD95 antigen was expressed on CD64(+) and CD64(-) monocytes at comparable levels, killing of CD64(-) monocytes by activating anti-Fas mAb was lower than of CD64(+) monocytes.

Place, publisher, year, edition, pages
1997. Vol. 27, no 9, 2358-2365 p.
Keyword [en]
Apoptosis, CD64-negative monocytes, cytotoxic T cell, dendritic cells, differential expression, enzyme-like protease, fas-mediated apoptosis, flow-cytometry, ifn-alpha, lymphocytes, macrophages, monoclonal-antibodies, presenting cells, recall antigen, resistance to killing
National Category
Biochemistry and Molecular Biology Immunology
URN: urn:nbn:se:liu:diva-87072DOI: 10.1002/eji.1830270934ISI: A1997YA32400033OAI: diva2:584806
Available from: 2013-01-09 Created: 2013-01-09 Last updated: 2013-09-03

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