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P2Z purinoreceptor ligation induces activation of caspases with distinct roles in apoptotic and necrotic alterations of cell death
Department of Internal Medicine I, Medical Clinics, Eberhard-Karls-University, Otfried-Müller-Str. 10, D-72076 Tübingen, Germany.
Department of Internal Medicine I, Medical Clinics, Eberhard-Karls-University, Otfried-Müller-Str. 10, D-72076 Tübingen, Germany.ORCID iD: 0000-0001-9518-1411
Department of Internal Medicine I, Medical Clinics, Eberhard-Karls-University, Otfried-Müller-Str. 10, D-72076 Tübingen, Germany.
Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology, Ghent, Belgium.
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1999 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 447, no 1, 71-75 p.Article in journal (Refereed) Published
Abstract [en]

Myeloic cells express a peculiar surface receptor for extracellular ATP, called the P2Z/P2X(7) purinoreceptor, which is involved in cell death signalling. Here, we investigated the role of caspases, a family of proteases implicated in apoptosis and the cytokine secretion. We observed that extracellular ATP induced the activation of multiple caspases including caspase-1, -3 and -8, and subsequent cleavage of the caspase substrates PARP and Iamin B. Using caspase inhibitors, it was found that caspases were specifically involved in ATP-induced apoptotic damage such as chromatin condensation and DNA fragmentation, In contrast, inhibition of caspases only marginally affected necrotic alterations and cell death proceeded normally whether or not nuclear damage was blocked. Our results therefore suggest that the activation of caspases by the P2Z receptor is required for apoptotic but not necrotic alterations of ATP-induced cell death. (C) 1999 Federation of European Biochemical Societies.

Place, publisher, year, edition, pages
Elsevier, 1999. Vol. 447, no 1, 71-75 p.
Keyword [en]
Apoptosis, atp, caspase, extracellular atp, flice, il-1-beta, necrosis, p2x(7), p2z, Proteasome, purinoreceptor, receptor, release, thymocyte apoptosis
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-87029DOI: 10.1016/S0014-5793(99)00270-7ISI: 000079428600017PubMedID: 10218585OAI: oai:DiVA.org:liu-87029DiVA: diva2:584265
Available from: 2013-01-08 Created: 2013-01-08 Last updated: 2017-12-06

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