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Functional characterization of DNase X, a novel endonuclease expressed in muscle cells
Department of Immunology and Cell Biology, University of Münster, Münster, and German Cancer Research Center, Division of Molecular Genome Analysis, Heidelberg, Germany .ORCID iD: 0000-0001-9518-1411
Department of Immunology and Cell Biology, University of Münster, Münster, and German Cancer Research Center, Division of Molecular Genome Analysis, Heidelberg, Germany .
German Cancer Research Center, Division of Molecular Genome Analysis, Heidelberg, Germany .
Department of Immunology and Cell Biology, University of Münster, Münster.
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2000 (English)In: Biochemistry, ISSN 0006-2960, E-ISSN 1520-4995, Vol. 39, no 25, 7365-7373 p.Article in journal (Refereed) Published
Abstract [en]

The activation of endonucleases resulting in the degradation of genomic DNA is one of the most characteristic changes in apoptosis. Here, we report the characterization of a novel endonuclease, termed DNase X due to its X-chromosomal localization. The active nuclease is a 35 kDa protein with 39% identity to DNase I. When incubated with isolated nuclei, recombinant DNase X was capable of triggering DNA degradation at internucleosomal sites. Similarly to DNase I, the nuclease activity of DNase X was dependent on Ca2+ and Mg2+ and inhibited by Zn2+ ions or chelators of bivalent cations. Overexpression of DNase X caused internucleosomal DNA degradation and induction of cell death associated with increased caspase activation. Despite the presence of two potential caspase cleavage sites, DNase X was processed neither in vitro nor in vivo by different caspases. Interestingly, after initiation of apoptosis DNase X was translocated from the cytoplasm to the nuclear compartment and aggregated as a detergent-insoluble complex. Abundant expression of DNase X mRNA was detected in heart and skeletal muscle cells, suggesting that DNase X may be involved in apoptotic or other biological events in muscle tissues.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2000. Vol. 39, no 25, 7365-7373 p.
Keyword [en]
apoptotic endonuclease, caspase-activated dnase, death, epithelial-cells, fragmentation, human deoxyribonuclease-ii, human xq28, molecular-cloning, protein, rat parotid-gland
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Biochemistry and Molecular Biology
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URN: urn:nbn:se:liu:diva-87019DOI: 10.1021/bi000158wISI: 000087918200005PubMedID: 10858283OAI: oai:DiVA.org:liu-87019DiVA: diva2:584251
Available from: 2013-01-08 Created: 2013-01-08 Last updated: 2017-12-06

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Los, Marek Jan

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