Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Myeloid-related protein-14 contributes to protective immunity in gram-negative pneumonia derived sepsis
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology. Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology.
Show others and affiliations
2012 (English)In: PLoS Pathogens, ISSN 1553-7374, Vol. 8, no 10, e1002987- p.Article in journal (Refereed) Published
Abstract [en]

Klebsiella (K.) pneumoniae is a common cause of pneumonia-derived sepsis. Myeloid related protein 8 (MRP8, S100A8) and MRP14 (S100A9) are the most abundant cytoplasmic proteins in neutrophils. They can form MRP8/14 heterodimers that are released upon cell stress stimuli. MRP8/14 reportedly exerts antimicrobial activity, but in acute fulminant sepsis models MRP8/14 has been found to contribute to organ damage and death. We here determined the role of MRP8/14 in K. pneumoniae sepsis originating from the lungs, using an established model characterized by gradual growth of bacteria with subsequent dissemination. Infection resulted in gradually increasing MRP8/14 levels in lungs and plasma. Mrp14 deficient (mrp14(-/-)) mice, unable to form MRP8/14 heterodimers, showed enhanced bacterial dissemination accompanied by increased organ damage and a reduced survival. Mrp14(-/-) macrophages were reduced in their capacity to phagocytose Klebsiella. In addition, recombinant MRP8/14 heterodimers, but not MRP8 or MRP14 alone, prevented growth of Klebsiella in vitro through chelation of divalent cations. Neutrophil extracellular traps (NETs) prepared from wildtype but not from mrp14(-/-) neutrophils inhibited Klebsiella growth; in accordance, the capacity of human NETs to kill Klebsiella was strongly impaired by an anti-MRP14 antibody or the addition of zinc. These results identify MRP8/14 as key player in protective innate immunity during Klebsiella pneumonia.

Place, publisher, year, edition, pages
San Fransisco: Public Library of Science , 2012. Vol. 8, no 10, e1002987- p.
National Category
Microbiology
Identifiers
URN: urn:nbn:se:umu:diva-62801DOI: 10.1371/journal.ppat.1002987ISI: 000310530300042PubMedID: 23133376OAI: oai:DiVA.org:umu-62801DiVA: diva2:581087
Available from: 2012-12-28 Created: 2012-12-18 Last updated: 2013-01-30Bibliographically approved

Open Access in DiVA

fulltext(2993 kB)212 downloads
File information
File name FULLTEXT02.pdfFile size 2993 kBChecksum SHA-512
155b77a5375f8e9a5bff623d3bdab18426e414055d1c70c184c052d33e03bb6ae0eb84fca2b14415d401b691f9e062851ab375939a9159f028f91639d2fc9346
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Urban, Constantin FRöhm, Marc
By organisation
Clinical BacteriologyMolecular Infection Medicine Sweden (MIMS)
Microbiology

Search outside of DiVA

GoogleGoogle Scholar
Total: 212 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 195 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf