Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Ghrelin and Obestatin in Human Neuroendocrine Tumors: Expression and Effect on Obestatin Levels after Food Intake
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Endocrine Oncology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Endocrine Oncology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Clinical Nutrition and Metabolism.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Biochemical endocrinology.
Show others and affiliations
2013 (English)In: Neuroendocrinology, ISSN 0028-3835, E-ISSN 1423-0194, Vol. 97, no 4, 291-299 p.Article in journal (Refereed) Published
Abstract [en]

Background:

Ghrelin and obestatin are derived from the same peptide hormone precursor and are mainly produced by the gastric mucosa. Ghrelin is involved in many biological processes, whereas the physiological function of obestatin needs further investigation. The aims of the present study were to establish the incidence of ghrelin- and obestatin-immunoreactive cells in a comprehensive panel of human neuroendocrine tumors (NETs) and to investigate if blood obestatin concentrations are influenced during a standardized meal stimulation test in healthy individuals and patients with NETs.

Materials and Methods:

The expression of ghrelin and obestatin was investigated in NETs (n = 149) and other endocrine-related disorders (n = 3) using immunohistochemistry with specific polyclonal antibodies. Coexpression of the peptides was evaluated by double immunofluorescence. Concentrations of obestatin in blood were measured during a meal test in 6 healthy individuals and 5 patients with pancreatic NETs.

Results:

Ghrelin and obestatin were expressed in 14/152 and 19/152 tumor tissues, respectively, mainly representing NETs of foregut origin and in pancreatic tissue from a nesidioblastosis patient. Double immunofluorescence staining showed colocalization of the peptides. During the meal test, obestatin levels in blood were unchanged in all patients but decreased significantly in the healthy individuals.

Conclusion:

Only a minority of NETs express ghrelin and obestatin. However, analysis of patients with tumors originating from tissues that express the peptides in normal conditions could be of importance. The results from the meal test indicate that the hormone levels are affected by food intake in healthy individuals, whereas obestatin levels remained unchanged in pancreatic NET patients.

Place, publisher, year, edition, pages
2013. Vol. 97, no 4, 291-299 p.
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:uu:diva-188786DOI: 10.1159/000345366ISI: 000320166900001PubMedID: 23147274OAI: oai:DiVA.org:uu-188786DiVA: diva2:579188
Available from: 2012-12-19 Created: 2012-12-19 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

fulltext(899 kB)233 downloads
File information
File name FULLTEXT01.pdfFile size 899 kBChecksum SHA-512
2d2fba7716674ae526de590f8babff029c8d0c8899672d18f976db7d8f8e691f979f99ae578991da02d86ffb58bcbdd31809abbbb63d952aeb4b1d263cff4ffd
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Grönberg, MalinTsolakis, Apostolos VHolmbäck, UlfStridsberg, MatsGrimelius, LarsJanson, Eva T

Search in DiVA

By author/editor
Grönberg, MalinTsolakis, Apostolos VHolmbäck, UlfStridsberg, MatsGrimelius, LarsJanson, Eva T
By organisation
Endocrine OncologyClinical Nutrition and MetabolismBiochemical endocrinologyMolecular and Morphological Pathology
In the same journal
Neuroendocrinology
Clinical Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 233 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 561 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf