Paralemmin-1 is over-expressed in estrogen-receptor positive breast cancers
2012 (English)In: Cancer Cell International, ISSN 1475-2867, Vol. 12, 17- p.Article in journal (Refereed) Published
Background: Paralemmin-1 is a phosphoprotein lipid-anchored to the cytoplasmic face of membranes where it functions in membrane dynamics, maintenance of cell shape, and process formation. Expression of paralemmin-1 and its major splice variant (Delta exon 8) as well as the extent of posttranslational modifications are tissue-and development-specific. Paralemmin-1 expression in normal breast and breast cancer tissue has not been described previously. Results: Paralemmin-1 mRNA and protein expression was evaluated in ten breast cell lines, 26 primary tumors, and 10 reduction mammoplasty (RM) tissues using real time RT-PCR. Paralemmin-1 splice variants were assessed in tumor and RM tissues using a series of primers and RT-PCR. Paralemmin-1 protein expression was examined in cell lines using Western Blots and in 31 ductal carcinomas in situ, 65 infiltrating ductal carcinomas, and 40 RM tissues using immunohistochemistry. Paralemmin-1 mRNA levels were higher in breast cancers than in RM tissue and estrogen receptor (ER)-positive tumors had higher transcript levels than ER-negative tumors. The Delta exon 8 splice variant was detected more frequently in tumor than in RM tissues. Protein expression was consistent with mRNA results showing higher paralemmin-1 expression in ER-positive tumors. Conclusions: The differential expression of paralemmin-1 in a subset of breast cancers suggests the existence of variation in membrane dynamics that may be exploited to improve diagnosis or provide a therapeutic target.
Place, publisher, year, edition, pages
2012. Vol. 12, 17- p.
Paralemmin-1, Breast cancer, PALM, Estrogen receptor, Tissue microarrays, Splice variants
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-188574DOI: 10.1186/1475-2867-12-17ISI: 000311333000001OAI: oai:DiVA.org:uu-188574DiVA: diva2:578435