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Visualization and analysis of vascular receptors using confocal laser scanning microscopy and fluorescent ligands
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
2012 (English)In: Receptor Binding Techniques / [ed] Anthony P. Davenport, New York: Humana Press, 2012, Vol. 897, 95-107 p.Chapter in book (Refereed)
Abstract [en]

The use of fluorescent ligands to analyze receptor distribution is increasing in popularity. This is due to the ever growing number of fluorescent ligands and the increased sensitivity of microscope-based technologies. Image-analysis methods have advanced to a stage where quantification of fluorescent signals is relatively simple (if used appropriately). In this chapter we describe a method of analyzing the 2D and 3D distribution of fluorescent ligands in segments of blood vessels. In addition, we introduce the issues surrounding the accurate analysis of colocalization of two different fluorescent ligands.

Place, publisher, year, edition, pages
New York: Humana Press, 2012. Vol. 897, 95-107 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 897
National Category
Cell Biology
URN: urn:nbn:se:uu:diva-188515DOI: 10.1007/978-1-61779-909-9_5PubMedID: 22674162ISBN: 978-1-61779-908-2OAI: diva2:578049
Available from: 2013-01-03 Created: 2012-12-17 Last updated: 2013-01-03Bibliographically approved

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Parmryd, Ingela
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