Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Low-medium resolution HLA-DQ2/DQ8 typing for coeliac disease predisposition analysis by colorimetric assay
Universitat Rovira i Virgili, Spain.
Universitat Rovira i Virgili, Spain. (Biosensors and Bioelectronics)ORCID iD: 0000-0001-6889-0351
Universitat Rovira i Virgili, Spain.
(Universitat Rovira i Virgili, Spain)
Show others and affiliations
2012 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 403, no 3, 807-819 p.Article in journal (Refereed) Published
Abstract [en]

Coeliac disease is an inflammation of the small intestine, occurring in genetically susceptible individuals triggered by the ingestion of gluten. Human Leukocyte Antigens (HLA) DQ2 and DQ8 gene have been identified as key genetic factors in coeliac disease as they are presented in almost 100 % of the patients. These genes are encoded by the combination of certain alleles in the DQA and DQB region of chromosome 6. Specifically, DQA1*05:01 and DQB1*02:01 alleles for serologically defined leukocyte antigen DQ2 cis, DQA1*05:05 and DQB1*02:02 for DQ2 trans and DQA1*03:01 and DQB1*03:02 alleles for the DQ8. Specific identification of these alleles is a challenge due to the high number of alleles that have been identified so far: 46 in the DQA region and 160 in the DQB region (as of IMGT/HLA Database 10/2011 release). In the reported work, the development of a multiplex colorimetric assay for the low to medium HLA typing of the DQ2 and DQ8 genes is presented. The optimisation of probe design and assay conditions, performed by both surface plasmon resonance and enzyme-linked oligonucleotide assay, are reported. Finally, the performances of the developed typing platform were validated by the analysis of real patient samples and HLA typing, compared with those obtained using hospital based typing technology and an excellent correlation obtained.

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2012. Vol. 403, no 3, 807-819 p.
Keyword [en]
Coeliac disease; HLA typing; Sequence specific oligonucleotide probes; SPR; ELONA
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-86352DOI: 10.1007/s00216-012-5898-6OAI: oai:DiVA.org:liu-86352DiVA: diva2:576783
Funder
EU, FP7, Seventh Framework Programme
Available from: 2012-12-18 Created: 2012-12-13 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

fulltext(275 kB)1409 downloads
File information
File name FULLTEXT02.pdfFile size 275 kBChecksum SHA-512
1720997a28ba67c09361b98157a545c15c86e993ad2325796c1027a460d4590785cba94c14aca20b6646db66c088fbd2eddb19aee76ec04e83a59a16f98c6c18
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Beni, Valerio
In the same journal
Analytical and Bioanalytical Chemistry
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
Total: 1409 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 179 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf