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Stripe Based CTF Gradient correction
KTH, School of Technology and Health (STH). (Hans Hebert Group)
2012 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Stripe Baserad CTF Gradient Korrigering (Swedish)
Abstract [en]

Abstract

Structure of membrane protiens can be determined by diffirent techniques.Electron Crystallography is one of the commonly used techniques to determine their structure in atomic or near atomic resolution.Due to the crystal disorder and poor CTF correction techniques the resolution obtained from this technique is not ideal.To push the resolution to the ideal, single particle refinement with local averaging for crystal disorder and improved CTF correction methodology for tilted data sets has been applied.With this approach applied to microsomal glutathione S-transferase 1(MGST1) membrane Protien data set comparable resolution with fewer data sets to the previous reconstruction has been achieved.

Place, publisher, year, edition, pages
2012. , p. 45
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-105882OAI: oai:DiVA.org:kth-105882DiVA, id: diva2:572719
Subject / course
Biotechnology
Educational program
Master of Science -Medical Imaging
Presentation
2012-10-18, 15:13 (English)
Uppsok
Technology
Supervisors
Examiners
Available from: 2019-09-11 Created: 2012-11-28 Last updated: 2019-09-11Bibliographically approved

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Stripe Based CTF Gradient Correction(1730 kB)2 downloads
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CiteExportLink to record
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  • apa
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Output format
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