Influence of poly(N-isopropylacrylamide)-CNT-polyaniline three-dimensional electrospun microfabric scaffolds on cell growth and viability
2013 (English)In: Biopolymers, ISSN 0006-3525, E-ISSN 1097-0282, Vol. 99, no 5, 334-341 p.Article in journal (Refereed) Published
This study investigates the effect on: 1) the bulk surface; and 2) the three-dimensional non-woven microfabric scaffolds of poly(N-isopropylacylamide)-CNT-polyaniline on growth and viability of mice fibroblast cells L929. The poly(N-isopropylacylamide)-CNT-polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, nonwoven microfabric scaffolds. The electrospun microfabrics were assembled in regular three-dimensional scaffolds with OD: 400-500 mm; L: 6-20 cm. Mice fibroblast cells L929 were seeded on the both poly(N-isopropylacylamide)-CNT-polyaniline bulk surface as well as non-woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N-isopropylacylamide)-CNT-polyaniline non-woven microfabric matrices in compare to poly(N-isopropylacylamide)-CNT-polyaniline bulk and commercially available Matrigel™ even with a range of cell lines up to 168 h. Temperature dependent cells detachment behaviour was observed on the poly(N-isopropylacylamide)-CNT-polyaniline scaffolds by varying incubation at below lower critical solution temperature (LCST) of poly(N-isopropylacylamide). The results suggest that poly(N-isopropylacylamide)-CNT-polyaniline non-woven microfabrics could be used as a smart matrices for applications in tissue engineering.
Place, publisher, year, edition, pages
John Wiley & Sons, 2013. Vol. 99, no 5, 334-341 p.
smart tissue engineering scaffolds, conducting polymers, carbon nanotubes, responsive-polymers, biocompatible conducting matrices.
IdentifiersURN: urn:nbn:se:liu:diva-85583DOI: 10.1002/bip.22170ISI: 000315300100006OAI: oai:DiVA.org:liu-85583DiVA: diva2:571857
ProjectsEuropean Commission FP7 (PIIF-GA-2009-254955), JSPS, JST-CREST and MEXT
FunderEU, FP7, Seventh Framework Programme, PIIF-GA-2009- 254955